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Team:The University of Alberta/29 May 2008
From 2008.igem.org
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==Volunteers Scheduled for Today== | ==Volunteers Scheduled for Today== | ||
| - | + | 130-4 NK<br> | |
4-8 AH<br> | 4-8 AH<br> | ||
5-9 KR<br> | 5-9 KR<br> | ||
6-9 DL | 6-9 DL | ||
| + | |||
| + | ==On this Episode of "What we Did in the Lab Today"== | ||
| + | '''Chris''': | ||
| + | * Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason): | ||
| + | ** B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1) | ||
| + | ** B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase | ||
| + | ** B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase | ||
| + | ** B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase | ||
| + | ** B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase | ||
| + | ** B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon | ||
| + | ** B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far. | ||
| + | I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)<br> | ||
| + | |||
| + | '''Jason''' | ||
| + | * Researched the GAA promoter that we could used for our ERE. | ||
| + | **Designed a Possible BioBrick | ||
| + | *Learned how to run a protien gel | ||
| + | |||
| + | '''Tune in Later for the Exciting conclusion of "What we did in the lab today"''' | ||
Latest revision as of 18:49, 29 May 2008
Volunteers Scheduled for Today
130-4 NK
4-8 AH
5-9 KR
6-9 DL
On this Episode of "What we Did in the Lab Today"
Chris:
- Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
- B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
- B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
- B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
- B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
- B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
- B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
- B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.
I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)
Jason
- Researched the GAA promoter that we could used for our ERE.
- Designed a Possible BioBrick
- Learned how to run a protien gel
Tune in Later for the Exciting conclusion of "What we did in the lab today"
