Team:The University of Alberta/29 May 2008

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Latest revision as of 18:49, 29 May 2008

Volunteers Scheduled for Today

130-4 NK
4-8 AH
5-9 KR
6-9 DL

On this Episode of "What we Did in the Lab Today"

Chris:

Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
- B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
- B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
- B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
- B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
- B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
- B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
- B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.

I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)

Jason

Researched the GAA promoter that we could used for our ERE.
- Designed a Possible BioBrick
Learned how to run a protien gel

Tune in Later for the Exciting conclusion of "What we did in the lab today"

@@ Line 8: / Line 8: @@
 ==On this Episode of "What we Did in the Lab Today"==
 '''Chris''':
-* Got sequencing results on B0043 back. Right now, the sequence files for only the first two genes in the operon have been put in the IGEM folder so I can only check to see if those two are right:
+* Got sequencing results on B0043 back. I aligned them with the sequences from last year to see what they were (just doing a Blast on the optimized sequences doesnt seem to work for some reason):
-** B0043+1 (the first gene) seems to be Enoyl-CoA Hydratase
+** B0043-VF (the first gene) seems to be ß-Hydroxy Butyryl Co-A Dehydrogenase (1)
-** B0043+2 (the second gene) seems to be Butyryl-CoA Dehydrogenase
+** B0043+1 (the second gene) seems to be Enoyl-CoA Hydratase
-Are these in the correct order? (James thinks theyre supposed to be the other way around). I dont know much about last year's project so I can't say.
+** B0043+2 (the third gene) seems to be Butyryl-CoA Dehydrogenase
+** B0043+3 (the fourth gene) seems to be Butyraldehyde Dehydrogenase
+** B0043+4 (the fourth gene) seems to be Butanol Dehydrogenase
+** B0043+5 (the fifth gene) aligned with nothing, which is good because there was no 5th gene in the operon
+** B0043-VR (sequencing from reverse) aligned with part of Butanol Dehydrogenase, which confirms that it is the last gene in the operon so far.
+I dunno if these are in the correct order but I don't think it really matters. As long as the enzymes are being produced and have the expected activity, things should be fine (correct me if I'm wrong!)<br>
 '''Jason'''
 * Researched the GAA promoter that we could used for our ERE.
@@ Line 17: / Line 23: @@
 *Learned how to run a protien gel
-'''Tune in Later for the Exciting conclusion of "What we did in the lab today"
+'''Tune in Later for the Exciting conclusion of "What we did in the lab today"'''