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Team:Hawaii/Notebook/2008-08-12
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Test of lab's RE (cont.)=== :<strong>Grace</strong> [[Image:081208enzymetest.jpg|right|thumb|200px|EtBr stained 1.2% ag...) |
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:* Colony PCR of transformants to check for correct insert | :* Colony PCR of transformants to check for correct insert | ||
| + | ::* Ligated slr, pilA with GFPf (ES digested) instead of GFPf (XP digested). Still got colonies. Hm... | ||
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===3A assembly=== | ===3A assembly=== | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
Revision as of 21:41, 12 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Test of lab's RE (cont.)
- Grace
- Ran RE digests from yesterday on a 1.2% agarose gel
Verified transformants
- Grace
| Construct | Colony forming units |
|---|---|
| GFP+B0015(tt) | 1 |
| GFPf+B0015(tt) | 52 |
| slr1+GFPf | 56 |
| pilA+GFPf | 43 |
- Colony PCR of transformants to check for correct insert
- Ligated slr, pilA with GFPf (ES digested) instead of GFPf (XP digested). Still got colonies. Hm...
3A assembly
- Grace
- Ligated p+r with g onto pSB1A2
- pnir+rbs with GFP
- plac+rbs with GFP
- Transformed into DH5α
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
