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Team:Hawaii/Notebook/2008-08-13
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of Transformants=== [[Image:081308colonyPCR.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95V f...) |
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| - | :* Colony PCR of transformants | + | :* Colony PCR of transformants and restreaked colonies from yesterday |
::* Ran on 2% agarose gel at 95V for 1 hour | ::* Ran on 2% agarose gel at 95V for 1 hour | ||
Revision as of 05:41, 13 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of Transformants
- Grace
| Construct | Colony forming units |
|---|---|
| slr1 + GFPf | |
| pilA + GFPf | |
| nir+rbs + GFP | |
| nir+rbs + slr1 | |
| nir+rbs + pilA | |
| plac+rbs + GFP | |
| plac+rbs + slr1 | |
| plac+rbs + pilA |
- Colony PCR of transformants and restreaked colonies from yesterday
- Ran on 2% agarose gel at 95V for 1 hour
Drylab Work
Sequencing
- Grace
- Checked sequencing results returned from CORE Hawaii
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
