Team:Hawaii/Notebook/2008-08-13

From 2008.igem.org

(Difference between revisions)
(→Verification of Transformants)
(→Verification of Transformants)
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| align=center|slr1 + GFPf
| align=center|slr1 + GFPf
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| align=center| 69 + 1 cluster of colonies
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| align=center|pilA + GFPf
| align=center|pilA + GFPf
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| align=center|17
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| align=center|nir+rbs + GFP
| align=center|nir+rbs + GFP
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| align=center|
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| align=center|0
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| align=center|nir+rbs + slr1
| align=center|nir+rbs + slr1
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| align=center|0
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| align=center|nir+rbs + pilA
| align=center|nir+rbs + pilA
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| align=center|1
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| align=center|plac+rbs + GFP
| align=center|plac+rbs + GFP
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| align=center|
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| align=center|0
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| align=center|plac+rbs + slr1
| align=center|plac+rbs + slr1
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| align=center|
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| align=center|0
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| align=center|plac+rbs + pilA
| align=center|plac+rbs + pilA
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:* Colony PCR of transformants and restreaked colonies from yesterday
:* Colony PCR of transformants and restreaked colonies from yesterday

Revision as of 18:14, 13 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Verification of Transformants

EtBr stained 2% agarose gel ran at 95V for 1 hour. Five microliters of PCR reaction were loaded into each well.
Grace
Construct Colony forming units
slr1 + GFPf 69 + 1 cluster of colonies
pilA + GFPf 17
nir+rbs + GFP 0
nir+rbs + slr1 0
nir+rbs + pilA 1
plac+rbs + GFP 0
plac+rbs + slr1 0
plac+rbs + pilA 0
  • Colony PCR of transformants and restreaked colonies from yesterday
  • Ran on 2% agarose gel at 95V for 1 hour

Drylab Work

Sequencing

Grace
  • Checked sequencing results returned from CORE Hawaii


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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