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Team:Hawaii/Notebook/2008-08-13
From 2008.igem.org
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:* Colony PCR of transformants and restreaked colonies from yesterday | :* Colony PCR of transformants and restreaked colonies from yesterday | ||
| - | ::* Ran on 2% agarose gel at 95V for 1 hour | + | ::* Ran on 2.5% agarose gel at 95V for 1 hour |
== Drylab Work == | == Drylab Work == | ||
Revision as of 18:14, 13 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of Transformants
- Grace
| Construct | Colony forming units |
|---|---|
| slr1 + GFPf | 69 + 1 cluster of colonies |
| pilA + GFPf | 17 |
| nir+rbs + GFP | 0 |
| nir+rbs + slr1 | 0 |
| nir+rbs + pilA | 1 |
| plac+rbs + GFP | 0 |
| plac+rbs + slr1 | 0 |
| plac+rbs + pilA | 0 |
- Colony PCR of transformants and restreaked colonies from yesterday
- Ran on 2.5% agarose gel at 95V for 1 hour
Drylab Work
Sequencing
- Grace
- Checked sequencing results returned from CORE Hawaii
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
