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Team:Hawaii/Notebook/2008-08-15
From 2008.igem.org
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===Reconstruction of BB-pRl1383a=== | ===Reconstruction of BB-pRl1383a=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | + | [[Image:081508REdigest.jpg|right|thumb|200px|EtBr stained 1% agarose gel ran at 72V for ... Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.]] | |
:* PCR amplification of J33207 | :* PCR amplification of J33207 | ||
::* ExoSAP'd PCR product | ::* ExoSAP'd PCR product | ||
Revision as of 22:45, 15 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Reconstruction of BB-pRl1383a
- Grace
- PCR amplification of J33207
- ExoSAP'd PCR product
- RE digested of J33207 and BB-pRL1383a (w/ GFP)
- Ran digests on a 1% agarose gel
- Extracted from bands from gel
- Ligated J33207 w/ BB-pRL1383a
- Transformed into DH5α
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
- DH5α does not carry lacIq; therefore, IPTG is not neccessary to induce the lac promoter.
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
