Revision as of 01:51, 16 August 2008

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Wetlab work

Reconstruction of BB-pRL1383a

EtBr stained 1% agarose gel ran at 72V for ... Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.

Grace

PCR amplification of J33207

ExoSAP'd PCR product

RE digested of J33207 and BB-pRL1383a (w/ GFP)
Ran digests on a 1% agarose gel at 72V for 1.5 hours.

Ladder did not run well. Bands not resolving well.

Redid PCR and RE digests

Drylab Work

Name of Task

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

Discussion

DH5α does not carry lacI^q; therefore, IPTG is not neccessary to induce the lac promoter.
IPTG is necessary for induction in DB3.1 cells.

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 10: / Line 10: @@
 ::* ExoSAP'd PCR product
 :* RE digested of J33207 and BB-pRL1383a (w/ GFP)
-:* Ran digests on a 1% agarose gel
+:* Ran digests on a 1% agarose gel at 72V for 1.5 hours.
-:* Extracted from bands from gel
+::* Ladder did not run well. Bands not resolving well.
-:* Ligated J33207 w/ BB-pRL1383a
+:* Redid PCR and RE digests
-:* Transformed into DH5&alpha;
 == Drylab Work ==

Team:Hawaii/Notebook/2008-08-15

From 2008.igem.org