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| - | [[Team:Chiba |Home]] / [[Team:Chiba/Internal |Internal]] / [[Team:Chiba/News|おしらせ]] / [[Team:Chiba/jk|実験ログ]] / [[Team:Chiba/Study|勉強会ログ]] / [[Team:Chiba/Meeting|Meeting]] / [[Team:Chiba/BS |BS]] / [[Team:Chiba/kk |考えてることを共有しようのページ]] / [[Team:Chiba/Graveyard|Graveyard]] | + | [[Team:Chiba |Home]] / [[Team:Chiba/Internal |Internal]] / [[Team:Chiba/News|おしらせ]] / [[Team:Chiba/jk|実験ログ]] / [[Team:Chiba/Study|勉強会ログ]] / [[Team:Chiba/Meeting|Meeting]] / [[Team:Chiba/BS |BS]] / [[Team:Chiba/Graveyard|Graveyard]] |
| - | ----[ | + | ---- |
| | + | チーム名をクリックすれば、実験ノートへうつります。 |
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| | + | '''[[Team:Chiba/jk/α|α-Team]]''' |
| | + | *入力システム班 |
| | + | :*小林あおい Aoi Kobayashi |
| | + | :*香取崇広 Takahiro Katori |
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| - | ]
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| - | '''[[Team:Chiba/jk/a|α-Team]]''' | + | '''[[Team:Chiba/jk/β|β-Team]]''' |
| - | *シグナル分子システム作成班 | + | *送信システム班 |
| | :*冨永将大 Masahiro Tominaga | | :*冨永将大 Masahiro Tominaga |
| | + | :*井山佳美 Yoshimi Iyama |
| | :*川崎浩平 Kohei Kawasaki | | :*川崎浩平 Kohei Kawasaki |
| - | :*久保喬義 Takayoshi Kubo
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| - | :*杉山まい Mai Sugiyama
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| - | *調べたこと
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| - | :*phnolの溶解度8.3 g/100 ml(20℃)[http://en.wikipedia.org/wiki/Phenol [1]]
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| - | :*tyrosineからphenolを合成する反応[http://www.kegg.jp/dbget-bin/www_bget?rn:R00728 [R00728]]
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| - | :*tyrosineからphenolを合成する酵素：TPL(Tyrosine Phenol-Lyase)[http://www.genome.jp/dbget-bin/www_bget?enzyme+4.1.99.2 EC 4.1.99.2 ]
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| - | :*TPL(Tyrosine Phenol-Lyase)の原典
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| - | ::*Kumagai, H.,and H. Hideki.Tyrosine Phenol Lyase Purification Crystallization,and Properties :''Biol.Chem''.,'''245''',No.7,1767-1772,1970
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| - | :::TPL(''Escherichia intermedia'' A-21由来)の特性について.以下の項目について説明があります。
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| - | :::*stability
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| - | :::*stoichiometry
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| - | :::*sedimentation coefficient(20℃,water)= 7.77
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| - | :::*diffusion coefficient(20℃,water)=4.42×10<sup>-7</sup>cm<sup>2</sup>/sec
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| - | :::*Michaelis constant = 2.31×10<sup>-4</sup> M (基質はL-tyrosine)
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| - | :::*inhibitor(L-alanine) K<sub>i</sub>=6.53×10<sup>-3</sup> M
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| - | :::*inhibitor(phenol) K<sub>i</sub>=3.56×10<sup>-5</sup> M
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| - | :::･･･他
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| - | ::*Kumagai, H.,and H. Hideki.Tyrosine Phenol Lyase Coefector Requirements:''Biol.Chem''.,'''245''',No.7,1773-1777,1970
| + | '''[[Team:Chiba/jk/γ|γ-Team]]''' |
| - | :::TPLはピリドキサルリン酸(PLPA)がないと不活性である。また、最大活性を示すには、K<sup>+</sup>およびNH<sub>4</sub><sup>+</sup>が必要。Na<sup>+</sup>により不活性化。など、TPLの機構についての説明があります。
| + | *受信システム班 |
| - | :*Nick J. P. Wierckx,* Hendrik Ballerstedt, Jan A. M. de Bont, and Jan Wery.Engineering of Solvent-Tolerant Pseudomonas putide S12 for Bioproduction of Phenol from Glucose:''Appl. Environ. Microbiol.'','''71''',No.12,8221-8227,2005
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| - | ::グルコースからチロシン経由でpseudomonas putide S１２で２５時間後にフェノールを１．５ｍＭ生成。オクタノールの媒質二相で100時間後にフェノールを５８ｍＭ生成
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| - | :*tyrosine biosynthesis pathway [http://www.kegg.jp/dbget-bin/get_pathway?org_name=ppu&mapno=00400 Phenylalanine, tyrosine and tryptophan biosynthesis - Pseudomonas putida KT2440]
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| - | ::＊論文とは違う株です。
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| - | :*Milic Dalibor; Matkovic-Calogovic Dubravka; Demidkina Tatyana V; Kulikova Vitalia V; Sinitzina Nina I; Antson Alfred A.Structures of apo- and holo-tyrosine phenol-lyase reveal a catalytically critical closed conformation and suggest a mechanism for activation by K+ ions.''Biochemistry'','''45''',7544-7552,2006
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| - | ::TPLの触媒作用に重要なコンホメーションについて、およびフェノール生成反応のメカニズムについての記述があります。、
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| - | :*Gibson, J.M.,P. S. Thomas, J. D.Thomas, J. L. Baker, S. S. Chandran, M. K. Harrup, K. M. Draths, and J. W. Frost.Benzene-Free Synthesis of Phenol.''Angew.Chem.Int.Engle''.'''40''',1945-1948
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| - | ::フェノールをシキミ酸から合成。
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| - | ----
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| - | | + | |
| - | '''[[Team:Chiba/jk/b|β-Team]]''' | + | |
| - | *出力システム作成班 | + | |
| | :*福冨浩樹 Hiroki Fukutomi | | :*福冨浩樹 Hiroki Fukutomi |
| - | :*小林あおい Aoi Kobayashi | + | :*杉山まい Mai Sugiyama |
| - | :*香取崇広 Takahiro Katori
| + | |
| - | :*井山佳美 Yoshimi Iyama
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| - | | + | |
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| | ---- | | ---- |
| - | [[Team:Chiba |Home]] / [[Team:Chiba/Internal |Internal]] / [[Team:Chiba/News|おしらせ]] / [[Team:Chiba/jk|実験ログ]] / [[Team:Chiba/Study|勉強会ログ]] / [[Team:Chiba/Meeting|Meeting]] / [[Team:Chiba/BS |BS]] / [[Team:Chiba/kk |考えてることを共有しようのページ]] / [[Team:Chiba/Graveyard|Graveyard]] | + | [[Team:Chiba |Home]] / [[Team:Chiba/Internal |Internal]] / [[Team:Chiba/News|おしらせ]] / [[Team:Chiba/jk|実験ログ]] / [[Team:Chiba/Study|勉強会ログ]] / [[Team:Chiba/Meeting|Meeting]] / [[Team:Chiba/BS |BS]] / [[Team:Chiba/Graveyard|Graveyard]] |
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