Team:Paris/Modeling/f0

From 2008.igem.org

(Difference between revisions)
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-
[exprJ23101] = γ<sub>GFP<sub>*[GFP] = γ<sub>GFP<sub>*(''fluorescence'')*''prop''.
+
[exprJ23101] = γ<sub>GFP<sub>*[GFP] = γ<sub>GFP</sub>*(''fluorescence'')*''conversion''.
-
where
+
 
-
{|
+
{|border="1" style="text-align: center"
|param
|param
|signification
|signification
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|
|
|-
|-
-
|γ<sup>GFP</sup>
+
|γ<sub>GFP</sub>
 +
|dilution-degradation rate <br> of GFP
|s<sup>-1</sup>
|s<sup>-1</sup>
|
|
|-
|-
|[GFP]
|[GFP]
-
|s<sup>-1</sup>
+
|GFP concentration at steady-state
 +
|nM
|
|
 +
|-
 +
|(''fluorescence'')
 +
|value of the observed fluorescence
 +
|au
 +
|
 +
|-
 +
|''conversion''
 +
|conversion ration between <br> fluorescence and concentration
 +
|(1/79.429)
|}
|}

Revision as of 15:38, 19 August 2008

F0.jpg
In the exponential phase of growth, at the steady state, we have


[exprJ23101] = γGFP*[GFP] = γGFP*(fluorescence)*conversion.


param signification unit value
[exprJ23101] expression rate of
J23101 with RBS E0032
nM.s-1
γGFP dilution-degradation rate
of GFP
s-1
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au
conversion conversion ration between
fluorescence and concentration
(1/79.429)