Team:Paris/Modeling/f0
From 2008.igem.org
(Difference between revisions)
Line 2: | Line 2: | ||
- | [exprJ23101] = γ<sub>GFP<sub>*[GFP] = γ<sub>GFP<sub>*(''fluorescence'')*'' | + | [exprJ23101] = γ<sub>GFP<sub>*[GFP] = γ<sub>GFP</sub>*(''fluorescence'')*''conversion''. |
- | + | ||
- | {| | + | {|border="1" style="text-align: center" |
|param | |param | ||
|signification | |signification | ||
Line 16: | Line 16: | ||
| | | | ||
|- | |- | ||
- | |γ< | + | |γ<sub>GFP</sub> |
+ | |dilution-degradation rate <br> of GFP | ||
|s<sup>-1</sup> | |s<sup>-1</sup> | ||
| | | | ||
|- | |- | ||
|[GFP] | |[GFP] | ||
- | | | + | |GFP concentration at steady-state |
+ | |nM | ||
| | | | ||
+ | |- | ||
+ | |(''fluorescence'') | ||
+ | |value of the observed fluorescence | ||
+ | |au | ||
+ | | | ||
+ | |- | ||
+ | |''conversion'' | ||
+ | |conversion ration between <br> fluorescence and concentration | ||
+ | |(1/79.429) | ||
|} | |} |
Revision as of 15:38, 19 August 2008
In the exponential phase of growth, at the steady state, we have
[exprJ23101] = γGFP*[GFP] = γGFP*(fluorescence)*conversion.
param | signification | unit | value |
[exprJ23101] | expression rate of J23101 with RBS E0032 | nM.s-1 | |
γGFP | dilution-degradation rate of GFP | s-1 | |
[GFP] | GFP concentration at steady-state | nM | |
(fluorescence) | value of the observed fluorescence | au | |
conversion | conversion ration between fluorescence and concentration | (1/79.429) |