|
Method & Algorithm : ƒ0
= act_J23101
 Specific Plasmid Characterisation for ƒ0 According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, the experiment would give us
↓ Table of Values ↑
| param
| signification
| unit
| value
| comments
|
| (fluorescence)
| value of the observed fluorescence
| au
|
| mean value of ~20 measures
|
| conversion
| conversion ratio between
fluorescence and concentration
↓ gives ↓
| nM.au-1
| (1/79.429)
|
|
| [GFP]
| GFP concentration at steady-state
| nM
|
|
|
| γGFP
| dilution-degradation rate
of GFP(mut3b)
↓ gives ↓
| min-1
| 0.0198
| Only dilution :
Time Cell Division : 35 min.
|
| ƒ0
| activity of
J23101 with RBS E0032
| nM.min-1
|
|
|
|
That will directly give us ƒ0
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|
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