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Edinburgh/19 June 2008
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==== Thursday 19 June 08 ==== | ==== Thursday 19 June 08 ==== | ||
| - | * Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight. | + | * Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight. |
| - | '''Plate 1:''' JM109 transformation with E0240 eluted DNA. | + | ** '''Plate 1:''' JM109 transformation with E0240 eluted DNA. |
| - | '''Plate 2:''' JM109 transformation with J33207 plasmid DNA (control). | + | ** '''Plate 2:''' JM109 transformation with J33207 plasmid DNA (control). |
Revision as of 13:54, 20 August 2008
Week 1
Thursday 19 June 08
- Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight.
- Plate 1: JM109 transformation with E0240 eluted DNA.
- Plate 2: JM109 transformation with J33207 plasmid DNA (control).
