Edinburgh/28 June 2008

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==== Saturday 28 June 08 ====
==== Saturday 28 June 08 ====
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* '''Result of transformations:'''<br />
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* '''Result of transformations:'''
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'''Plate 3:''' 1 white; '''Plate 7:''' 5 white, 13 blue.<br />
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** '''Plate 3:''' 1 white; '''Plate 7:''' 5 white, 13 blue.
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'''Plate 4:''' no growth; '''Plate 8:''' no growth.<br />
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** '''Plate 4:''' no growth; '''Plate 8:''' no growth.
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'''Plate 5:''' 1 white; '''Plate 9:''' 3 white, 20 blue, possible signs of phage.<br />
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** '''Plate 5:''' 1 white; '''Plate 9:''' 3 white, 20 blue, possible signs of phage.
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'''Plate 6:''' maybe one tiny white; '''Plate 10:''' 7 white, 9 blue.<br />
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** '''Plate 6:''' maybe one tiny white; '''Plate 10:''' 7 white, 9 blue.
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The white colonies were transferred to fresh plates of the same medium:<br />
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* The white colonies were transferred to fresh plates of the same medium:
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'''Plate 11:''' possible pSB1A2+''dxs'' transformants.<br />
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** '''Plate 11:''' possible pSB1A2+''dxs'' transformants.
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'''Plate 12:''' possible Babel1+''glgC'' transformants.<br />
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** '''Plate 12:''' possible Babel1+''glgC'' transformants.
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'''Plate 13:''' possible Babel2+''glgC'' transformants.<br />
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** '''Plate 13:''' possible Babel2+''glgC'' transformants.
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The new plates were incubated at 37°C. The old plates were left at room temperature to see if any further colonies would appear.
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* The new plates were incubated at 37°C. The old plates were left at room temperature to see if any further colonies would appear.

Revision as of 14:18, 20 August 2008

Edinburgh iGEM 2008

 

Week 2

Saturday 28 June 08

  • Result of transformations:
    • Plate 3: 1 white; Plate 7: 5 white, 13 blue.
    • Plate 4: no growth; Plate 8: no growth.
    • Plate 5: 1 white; Plate 9: 3 white, 20 blue, possible signs of phage.
    • Plate 6: maybe one tiny white; Plate 10: 7 white, 9 blue.
  • The white colonies were transferred to fresh plates of the same medium:
    • Plate 11: possible pSB1A2+dxs transformants.
    • Plate 12: possible Babel1+glgC transformants.
    • Plate 13: possible Babel2+glgC transformants.
  • The new plates were incubated at 37°C. The old plates were left at room temperature to see if any further colonies would appear.
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