Edinburgh/19 June 2008
From 2008.igem.org
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
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+ | :::: '''[[Edinburgh/17_June_2008|< Previous Entry]]''' | ||
== Week 1 == | == Week 1 == | ||
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* Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight. | * Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight. | ||
** '''Plate 1:''' JM109 transformation with E0240 eluted DNA. | ** '''Plate 1:''' JM109 transformation with E0240 eluted DNA. | ||
- | ** '''Plate 2:''' JM109 transformation with J33207 plasmid DNA (control). | + | ** '''Plate 2:''' JM109 transformation with J33207 plasmid DNA (control).<br /> |
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+ | :::: '''[[Edinburgh/20_June_2008|Next Entry >]]''' |
Revision as of 15:00, 20 August 2008
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Week 1
Thursday 19 June 08
- Eluted DNA for BioBrick E0240 (GFP) from square 1001, well 4B and used this to transform half of tube J25; other half was transformed with 1.5μl of J33207 (Edinbrick1) as a control to test the competence of the cells. Plated 100 microlitres of each to LA+amb+BW. Incubate at 37°C overnight.
- Plate 1: JM109 transformation with E0240 eluted DNA.
- Plate 2: JM109 transformation with J33207 plasmid DNA (control).