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Edinburgh/18 August 2008
From 2008.igem.org
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| - | = Week 10 = | + | === Week 10 === |
| - | == Monday 18 August 08 == | + | ==== Monday 18 August 08 ==== |
*M144-M147: Minipreps of cenA(1,2,3,4). M148-M151: Minipreps of cex(1,2,3,4). M152-M155: Munipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG) | *M144-M147: Minipreps of cenA(1,2,3,4). M148-M151: Minipreps of cex(1,2,3,4). M152-M155: Munipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG) | ||
Revision as of 11:42, 21 August 2008
Week 10
Monday 18 August 08
- M144-M147: Minipreps of cenA(1,2,3,4). M148-M151: Minipreps of cex(1,2,3,4). M152-M155: Munipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG)
- Minipreps 144-155 were digested with EcoRI/PstI. This need running on a gel tomorrow. (AH)
- X11: Maxiprep of Zea mays isoamylase 1 (isa1+pZntr22). X12: Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)
- Combined biobricks:
L42: dxs+lims (M137) to appY (M82). L43: crtB+crtI (M109) to appY (M82). L44: pstA (M130) to lacB. (YAN)
- Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
- Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
