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Edinburgh/13 July 2008
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| - | ==== Sunday 13 July 08 | + | <html> |
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| + | {| align="left" | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
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| + | :::: '''[[Edinburgh/12_July_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 4 == | ||
| + | === Sunday 13 July 08 === | ||
* Signs of growth on ''Cellulomonas'' plates 28 and 29. | * Signs of growth on ''Cellulomonas'' plates 28 and 29. | ||
* '''Minipreps M25 to M30''' of possible rbs+''dxs'' clones from plate 34. | * '''Minipreps M25 to M30''' of possible rbs+''dxs'' clones from plate 34. | ||
| - | * '''PCR P12''' to obtain rbs+''dxs'' fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD. | + | * '''PCR P12''' to obtain rbs+''dxs'' fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD.<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/14_July_2008|Next Entry >]]''' | ||
Revision as of 12:09, 21 August 2008
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Week 4
Sunday 13 July 08
- Signs of growth on Cellulomonas plates 28 and 29.
- Minipreps M25 to M30 of possible rbs+dxs clones from plate 34.
- PCR P12 to obtain rbs+dxs fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD.
