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Edinburgh/Notebook/Minipreps
From 2008.igem.org
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'''M97 to M104:''' pSB1A2+''cenA'' (05.08.08: YAN)<br /> | '''M97 to M104:''' pSB1A2+''cenA'' (05.08.08: YAN)<br /> | ||
'''M105 to M108:''' pSB1A2+''cex'' (05.08.08: YAN)<br /> | '''M105 to M108:''' pSB1A2+''cex'' (05.08.08: YAN)<br /> | ||
| + | '''M109 to M112''': crtBI (11.08.08: OG)<br /> | ||
| + | '''M113 to M116''': glgc mutant 1+2 (11.08.08: OG)<br /> | ||
| + | '''M117 to M120''': glgc mutant 1+2+3 (11.08.08: OG)<br /> | ||
| + | '''M121 to M123''': pSB1A2-crtY (12.08.08: OG)<br /> | ||
| + | '''M124 to M126''': pSBA12-rbs+crtY (12.08.08: OG)<br /> | ||
| + | '''M127-M130''': pSB1A2-PcstA from plate 115 (13.08.08: OG)<br /> | ||
| + | '''M131-M134''': Minipreps of dxs+crtE (1,2,3,4) Note: M132 might be M136 (13.08.08: OG)<br /> | ||
| + | '''M135-M138''': Minipreps of dxs+lims (1,2,3,4) Note: M136 might be M132 (13.08.08: OG)<br /> | ||
| + | '''M139 to M143''': Minipreps of pSB1A2 +CrtE (1 to 4) Note: M142 and M143 both made from culture 4.(15.08.08: YAN/OG)<br /> | ||
| + | '''M144-M147''': pSB1A2+cenA from plate 129 - Methanol used in preparation rather than ethanol! (18.08.08: OG)<br /> | ||
| + | '''M148-M151''': pSB1A2+cex from plate 129. (18.08.08: OG)<br /> | ||
| + | '''M152-M155''': pSB1A2+dxs+crtE from plate 130 (18.08.08: OG)<br /> | ||
Revision as of 13:46, 22 August 2008
M1 to M6: pSB1A2+dxs transformants (plate 11). M2, M3, M4 and M6 all looked correct on gel 2. (30.06.08) - M2 was sequenced to confirm identity and was maxiprepped as X2.
M7: BABEL1+glgC transformant (plate 12). Did not look right on gel. (30.06.08)
M8 to M12: BABEL2+glgC transformants (plate 13). M10 and M11 both looked good on a gel, but sequencing showed that both had the insert in the reverse orientation. (30.06.08)
M13 to M18: pSB1A2+appY transformants. Gel 10 showed that M15 to M18, and possibly M14, all seemed to have inserts the right size. (09.07.08)
M19 to M24: BABEL2+glgC clones after mutation of EcoRI site 2. EcoRI digests on *Gel 11* suggest that *M19, M21 and M22* may have lost the EcoRI site. (10.07.08)
M25 to M30: possible rbs+dxs transformants from plate 34. (13.07.08)
M31 to M36: pSB1A2+crtB clones from plate 35. (14.07.08)
M37 to M42: pSB1A2+crtI clones from plate 36. (14.07.08)
M43 to M47: possible BABEL2+glgC double mutants (both EcoRI sites gone). (19.07.08)
M48: a possible pSB1A2+rbs+dxs clone. (19.07.08)
M49 to M54: pSB1A2-rbs+crtI
M55 to M60: PZntA promoter
M61 to M66: BABEL2+rbs+crtE
M67: rbs+crtB
M68 to M71: pSB1A2+glgC-mut1,2 (31.07.08: AM, YAN)
M72 to M75: pSB1A2+rbs+dxs (31.07.08: AM, YAN)
M76 to M79: BABEL2+crtE (31.07.08: AM, YAN)
M80 to M83: pSB1A2+rbs+appY (04/08/08: YAN and OG)
M84 to M85: pSB1A2+rbs+crtB (04/08/08: YAN and OG)
M86 to M90: pSB1A2+cenA (04/08/08: YAN and OG)
M91 to M96: pSB1A2+cex(04/08/08: YAN and OG)
M97 to M104: pSB1A2+cenA (05.08.08: YAN)
M105 to M108: pSB1A2+cex (05.08.08: YAN)
M109 to M112: crtBI (11.08.08: OG)
M113 to M116: glgc mutant 1+2 (11.08.08: OG)
M117 to M120: glgc mutant 1+2+3 (11.08.08: OG)
M121 to M123: pSB1A2-crtY (12.08.08: OG)
M124 to M126: pSBA12-rbs+crtY (12.08.08: OG)
M127-M130: pSB1A2-PcstA from plate 115 (13.08.08: OG)
M131-M134: Minipreps of dxs+crtE (1,2,3,4) Note: M132 might be M136 (13.08.08: OG)
M135-M138: Minipreps of dxs+lims (1,2,3,4) Note: M136 might be M132 (13.08.08: OG)
M139 to M143: Minipreps of pSB1A2 +CrtE (1 to 4) Note: M142 and M143 both made from culture 4.(15.08.08: YAN/OG)
M144-M147: pSB1A2+cenA from plate 129 - Methanol used in preparation rather than ethanol! (18.08.08: OG)
M148-M151: pSB1A2+cex from plate 129. (18.08.08: OG)
M152-M155: pSB1A2+dxs+crtE from plate 130 (18.08.08: OG)
