Latest revision as of 13:41, 23 August 2008

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Things we did today

EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.

Grace

Grace

Grace

Grace

Margaret

Margaret

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 4: / Line 4: @@
 == Wetlab work ==
 ===Verification of transformants===
-[[Image:082208colonypcr.jpg|right|thumb|300px|EtBr stained 2% agarose gel ran at 95v for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.]]
+[[Image:082208colonypcr.jpg|right|thumb|400px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Five microliters of PCR reaction were loaded into each lane.]]
 :<strong> Grace</strong>
 {|class=wikitable border=1 align=center
@@ Line 25: / Line 25: @@
 :* Colony PCR of transformants
 :* Ran PCR reaction on 2% agarose gel
+::* Can't tell 15bp difference between GFP and rbs+GFP
+:::* Restreaked single colony and inoculated for plasmid prep to confirm
+::* nir+rbs colonies #11-19 appear promising (larger than nir)
+:::* Restreaked colony #19 and inoculated for plasmid prep to confirm
 ===Plasmid prep of J33207===
@@ Line 36: / Line 40: @@
 :* GFP, GFPf, nir with EcoRI and SpeI
-== Drylab Work ==
+===Made LB+amp<sub>100</sub> plates===
+:<strong>Grace</strong>
-===Name of Task===
-:<strong> name of person/people who performed the task</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+===Ligation===
-:* e.g. read through papers, worked on proposal, etc.
+<strong>Margaret</strong>
+:*ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3
+===Omega interposon===
+:<strong>Margaret</strong>
+:*streak on Sm&Sp plate to confirm insertion.
 = Discussion =