Edinburgh/Notebook/Plates

From 2008.igem.org

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'''Plates 46-47:''' L16 transformation (rbs+''appY'' with Edinbrick1) (18.07.08) - No growth<br />
'''Plates 46-47:''' L16 transformation (rbs+''appY'' with Edinbrick1) (18.07.08) - No growth<br />
'''Plates 48-50:''' ''Cellulomonas fimi'' from overnight cultures made from plate 37. (24.07.08: AM)<br />
'''Plates 48-50:''' ''Cellulomonas fimi'' from overnight cultures made from plate 37. (24.07.08: AM)<br />
-
'''Plates 51/52:''' L19 Transforations (rbs\-_dxs_\-Edinbrick) (26/07/08: CF)<br />
+
'''Plates 51-52:''' L19 (pSB1A2+rbs+''dxs'') transformation. (26.07.08: CF) - No growth<br />
-
'''Plates 53/54:''' L20 Transforations (rbs\-_appY_\-Edinbrick) (26/07/08: CF)<br />
+
'''Plates 53-54:''' L20 (pSB1A2+rbs+''appY'') transformation. (26.07.08: CF) - No growth<br />
-
'''Plates 55/56:''' L21 Transforations (rbs\-_crtB_\-Edinbrick) (26/07/08: CF)<br />
+
'''Plates 55-56:''' L21 (pSB1A2+rbs+''crtB'') transformation. (26.07.08: CF) - No growth<br />
-
'''Plates 57/58:''' L22 Transforations (rbs\-_crtI_\-Edinbrick) (26/07/08: CF)<br />
+
'''Plates 57-58:''' L22 (pSB1A2+rbs+''crtI'') transformation. (26.07.08: CF)<br />
-
'''Plates 59/60:''' L23 Transforations (PzntA-Edinbrick) (26/07/08: CF)<br />
+
'''Plates 59-60:''' L23 (pSB1A2+pZntA) transformation. (26.07.08: CF)<br />
-
'''Plates 61/62:''' L24 Transforations (_crtE_\-pSB1A2 - from BABEL) (26/07/08: CF)<br />
+
'''Plates 61-62:''' L24 (pSB1A2+''crtE'' - from BABEL2) transformation. (26.07.08: CF)<br />
-
'''Plates 63/64:''' Repeat transformations of L20 (28/07/08: Yan, OG)<br />
+
'''Plates 63-64:''' Repeat transformation of L20 (pSB1A2+rbs+''appY''). (28.07.08: Yan, OG) - No growth<br />
-
'''Plates 65/66:''' Repeat transformations of L21 (28/07/08: Yan, OG)<br />
+
'''Plates 65-66:''' Repeat transformation of L21 (pSB1A2+rbs+''crtB''). (28.07.08: Yan, OG)<br />
-
'''Plates 67/68:''' glgc mutant 1&2 transformation (29.07.2008: Yan)<br />
+
'''Plates 67-68:''' L25 (pSB1A2+''glgC''-mut1,2) transformation. (29.07.2008: Yan)<br />
-
'''Plates 69/70:''' rbs+dxs, 100 and 900 µl (30.07.2008: CF)<br />
+
'''Plates 69-70:''' pSB1A2+rbs+''dxs'' ligation. (30.07.2008: CF)<br />
-
'''Plates 71/72:''' crtE coding sequence in Babel2, 100 and 900 µl (30.07.2008: CF)<br />
+
'''Plates 71-72:''' ligation of ''crtE'' to BABEL2. (30.07.2008: CF)<br />
-
'''Plate 73:''' Subs from Plate 67 (30.07.2008: CF)<br />
+
'''Plate 73:''' Subs from plate 67 (pSB1A2+''glgC''-mut1,2) (30.07.2008: CF)<br />
-
'''Plate 74:''' Subs from Plates 69-72 (30.07.2008: CF)<br />
+
'''Plate 74:''' Subs from plates 69-72 (pSB1A2+rbs+''dxs'' and BABEL2+''crtE'') (30.07.2008: CF)<br />
-
'''Plates 75/76:''' L26 (Edi-rbs\-_appY_) 100 microlitres/900 microlitres (31.07.2008: HX)<br />
+
'''Plates 75-76:''' L26 (pSB1A2+rbs+''appY'') transformation' (31.07.2008: HX)<br />
-
'''Plates 77/78:''' L27 (Edi-rbs\-_crtB_) 100 microlitres/900 microlitres (31.07.2008: HX)<br />
+
'''Plates 77-78:''' L27 (pSB1A2+rbs+''crtB'') transformation' (31.07.2008: HX)<br />
-
'''Plates 79/80:''' L28 (Edi\-_cenA_) 100 microlitres/900 microlitres (31.07.2008: HX)<br />
+
'''Plates 79-80:''' L28 (pSB1A2+''cenA'') transformation' (31.07.2008: HX)<br />
-
'''Plates 81/82:''' L29 (Edi\-_cex_) 100 microlitres/900 microlitres (31.07.2008: HX)<br />
+
'''Plates 81-82:''' L29 (pSB1A2+''cex'') transformation' (31.07.2008: HX)<br />
'''Plate 83:''' Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)<br />
'''Plate 83:''' Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)<br />
'''Plate 84:''' Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)<br />
'''Plate 84:''' Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)<br />

Revision as of 15:52, 26 August 2008

Edinburgh iGem 2008

 

Plates 1-2: transformation with a BioBrick from the Registry stock: failed. (18.06.08)
Plate 3: L1 (pSB1A2+dxs) transformation, 100μl. (27.06.08)
Plate 4: L2 (pSB1A2+appY) transformation, 100μl. (27.06.08) - Failed
Plate 5: L3 (BABEL1+glgC) transformation, 100μl. (27.06.08)
Plate 6: L4 (BABEL2+glgC) transformation, 100μl. (27.06.08)
Plate 7: L1 (pSB1A2+dxs) transformation, 900μl. (27.06.08)
Plate 8: L2 (pSB1A2+appY) transformation, 900μl. (27.06.08) - Failed
Plate 9: L3 (BABEL1+glgC) transformation, 900μl. (27.06.08)
Plate 10: L4 (BABEL2+glgC) transformation, 900μl. (27.06.08)
Plate 11: possible pSB1A2+dxs transformants. (28.06.08)
Plate 12: possible Babel1+glgC transformants. (28.06.08)
Plate 13: possible Babel2+glgC transformants. (28.06.08)
Plate 14: more subcultures from plates 5 and 6. (29.06.08)
Plates 15-16: retransformation of religated L2. Failed again. (03.07.08)
Plate 17: Pantoea ananatis for crt PCR reactions. (05.07.08)
Plates 18-19: L5 (pSB1A2+appY) transformation, 100μl and 900μl. (05.07.08)
Plate 20: subculture from plate 19. (06.07.08)
Plate 21: subcultures from plate 19. (07.07.08)
Plates 22-23: L6 (BABEL2+glgC-mut1) transformation. No colonies. (08.07.08)
Plates 24-25: L7 (BABEL2+glgC-mut2) transformation. (08.07.08)
Plates 26-27: subcultures from plates 24 and 25 (L7). (09.07.08)
Plates 28-29: Cellulomonas fimi from initial stock. (11.07.08)
Plates 30-31: possible pSB1A2+crtB (L9) transformants. (11.07.08)
Plates 32-33: possible pSB1A2+crtI-mut (L10) transformants. (11.07.08)
Plate 34: possible pSB1A2+rbs+dxs clones from Nimisha's plates. (11.07.08)
Plate 35: patches of possible pSB1A2+crtB transformants from Plate 30. (12.07.08)
Plate 36: patches of possible pSB1A2+crtI-mut transformants from Plates 32 and 33. (12.07.08)
Plate 37: subculture of C. fimi on NA with filter paper (cellulose). (15.07.08)
Plates 38-39: L15 transformation (pSB1A2+rbs+dxs). (15.07.08)
Plates 40-41: L13 transformation (BABEL2+glgC-mut1,2). (15.07.08)
Plates 42, 44 and 45: Patches of possible BABEL2+glgC-mut1,2 transformants - subcultures from plate 41. (17.07.08)
Plate 43: Patches of possible pSB1A2+rbs+dxs transformants - subcultures from plate 38 or 39. (17.07.08)
Plates 46-47: L16 transformation (rbs+appY with Edinbrick1) (18.07.08) - No growth
Plates 48-50: Cellulomonas fimi from overnight cultures made from plate 37. (24.07.08: AM)
Plates 51-52: L19 (pSB1A2+rbs+dxs) transformation. (26.07.08: CF) - No growth
Plates 53-54: L20 (pSB1A2+rbs+appY) transformation. (26.07.08: CF) - No growth
Plates 55-56: L21 (pSB1A2+rbs+crtB) transformation. (26.07.08: CF) - No growth
Plates 57-58: L22 (pSB1A2+rbs+crtI) transformation. (26.07.08: CF)
Plates 59-60: L23 (pSB1A2+pZntA) transformation. (26.07.08: CF)
Plates 61-62: L24 (pSB1A2+crtE - from BABEL2) transformation. (26.07.08: CF)
Plates 63-64: Repeat transformation of L20 (pSB1A2+rbs+appY). (28.07.08: Yan, OG) - No growth
Plates 65-66: Repeat transformation of L21 (pSB1A2+rbs+crtB). (28.07.08: Yan, OG)
Plates 67-68: L25 (pSB1A2+glgC-mut1,2) transformation. (29.07.2008: Yan)
Plates 69-70: pSB1A2+rbs+dxs ligation. (30.07.2008: CF)
Plates 71-72: ligation of crtE to BABEL2. (30.07.2008: CF)
Plate 73: Subs from plate 67 (pSB1A2+glgC-mut1,2) (30.07.2008: CF)
Plate 74: Subs from plates 69-72 (pSB1A2+rbs+dxs and BABEL2+crtE) (30.07.2008: CF)
Plates 75-76: L26 (pSB1A2+rbs+appY) transformation' (31.07.2008: HX)
Plates 77-78: L27 (pSB1A2+rbs+crtB) transformation' (31.07.2008: HX)
Plates 79-80: L28 (pSB1A2+cenA) transformation' (31.07.2008: HX)
Plates 81-82: L29 (pSB1A2+cex) transformation' (31.07.2008: HX)
Plate 83: Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)
Plate 84: Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)
Plate 85: Subs from Plate 80 (L28: Edi+cenA) (01.08.2008: AM)
Plate 86: Subs from Plates 81/82 (L29: Edi+cex) (01.08.2008: AM)
Plate 89/90: L30 (Self-ligation of P15) 100ml/900ml (06.08.2008: HX)
Plate 91/92: L31 (Double digestion of P36/P39 by OG) 100ml/900ml (06.08.2008: HX)
Plate 93: Subculture from Plate 56 (M67, pSB1A2-rbs\-_crtB_) (05.08.08: CF)
Plate 94: Subculture from Plate 89,90 (07.08.08: HX)
Plate 95: Subculture from Plate 92 (07.08.08: HX)
Plate 96: LB, ampicillin plate spread with subcultures from plate 43. (HX, 07.08.08)
Plate 97: LB, ampicillin, 2% glucose plate spread with subcultures from plate 43. (HX, 07.08.08)
Plates 98/99: Transformation of L32 (_crtB_/_crtI_) (08.08.08: HX)
Plate 100/101: Repeat transformation of L31 (Double digestion of P36/P39 by OG) (08.08.08: HX)
Plate 102: Patches from plate 99 (L32, crtBI) (10.08.08: CF)
Plate 103: Patches from plate 101 (L31, glgC double mutant+pSB1A2) (10.08.08: CF)
Plate 104/105: Transform L36 pSB1A2-crtY 100µl/900µl (10.08.08: CF)
Plate 106/107: Transform L37 pSB1A2-rbs-crtY 100µl/900µl (10.08.08: CF)
Plate 108: Subcultures from plates 105 and 106 (11.08.08: CF)
Plate 109/110: Transformation of L33 pSB1A2-PcstA 100µl/900µl (11.08.08: Yan)
Plate 111/112: Transformation of L34 dxs-crtE 100µl/900µl (11.08.08: Yan)
Plate 113/114: Transformation of L35 dxs-lims 100µl/900µl (11.08.08: Yan)
Plate 115: Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-lims)
Plates 116/117: Transformation of L38 (psb1A2\-_crtE_) (13.08.08: HX)
Plate 118: Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
Plate 119/120: Transformation of L40 (CenA+Edinbrick1) (14.08.08: YAN)
Plate 121/122: Transformation of L39 (Cex+Edinbrick1) (14.08.08: YAN)
Plate 123/124: Repeat to transform with L39 (cex+Edinbrick1)  (15.08.08: HX)
Plate 125/126: Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
Plate 127/128: Transform with L41 (dxs/crtE) (15.08.08: HX)
Plate 129: CenA & CeX white colonies from Plates 120 & 123-126 (16/08: CF)
Plate 130: dxs-CrtE white colonies from plate 127-128 (16/08: CF)
Plate 131/132: maize pENTR221 transformation, ISA1 Kan 10 (16/08: CF)
Plate 133/134: maize pENTR221 transformation, ISA2 Kan 10 (16/08: CF)
Plate 135: patches(colonies) from plates 131,133 (16/08: CF)
Plate 136/137: Transformation with L48 (dxs+Lims+Appy) (20.08.08: Yan)
Plate 138/139: Transformation with L49 (CrtBI+Appy) (20.08.08: YAN)
Plate 140/141: Transformation with Edinbrick1 only as control (20.08.08: YAN)

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