Team:Hawaii/Notebook/2008-08-25

From 2008.igem.org

(Difference between revisions)
(margaret)
(Things we did today)
 
(2 intermediate revisions not shown)
Line 12: Line 12:
:*verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation
:*verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation
-
== Drylab Work ==
+
===Verification of transformants (cont.)===
 +
[[Image:082508PCR.jpg|right|thumb|250px|EtBr stained 3% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.]]
 +
:<strong> Grace</strong>
-
===Name of Task===
+
:* PCR'd nir, nir+rbs, GFP, GFP+rbs
-
:<strong> name of person/people who performed the task</strong>
+
:* Ran on 3% agarose gel
-
:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+
===Restriction digest===
-
:* e.g. read through papers, worked on proposal, etc.
+
:<strong>Grace</strong>
 +
:* RE digested overnight:
 +
::* BB-pRL1383a and J33207 with XbaI and PstI
 +
::* C0012 with EcoRI and PstI (to isolate vector)
 +
::* nir with EcoRI and SpeI
 +
 +
===Ligation===
 +
:<strong>Krystle</strong>
 +
 +
:* Ligated overnight:
 +
::* plac + B0034 (both synthesized)
 +
::* plac + B0030 (both synthesized)
 +
::* gfpf (vector) + tt (synthesized)
 +
::* gfpf (re'd with E-S) + tt (synthesized)
= Discussion =
= Discussion =

Latest revision as of 03:05, 27 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Ligation & Transformation

Margaret
  • ligation of rep+pSB1A3 and P1+pSB1A3, transformation into DH5alpha cells

Colony PCR

Margaret

  • verified the colonies from the (pLac+B0030) ligation and transformation. no transformants for the B0034 ligation

Verification of transformants (cont.)

EtBr stained 3% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.
Grace
  • PCR'd nir, nir+rbs, GFP, GFP+rbs
  • Ran on 3% agarose gel

Restriction digest

Grace
  • RE digested overnight:
  • BB-pRL1383a and J33207 with XbaI and PstI
  • C0012 with EcoRI and PstI (to isolate vector)
  • nir with EcoRI and SpeI

Ligation

Krystle
  • Ligated overnight:
  • plac + B0034 (both synthesized)
  • plac + B0030 (both synthesized)
  • gfpf (vector) + tt (synthesized)
  • gfpf (re'd with E-S) + tt (synthesized)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]