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Team:Hawaii/Notebook/2008-08-27
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r=== :<strong>Grace</strong> :* Ran RE digested products on gel :* Extracted nir, C0012 vector, BB-p...) |
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== Wetlab work == | == Wetlab work == | ||
===Construction of p+r=== | ===Construction of p+r=== | ||
| + | [[Image:082708REdigests.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 60V for 2.25 hours. Thirty microliters of RE digested DNA were loaded into each well.]] | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
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::* pSB1A2 (negative control) | ::* pSB1A2 (negative control) | ||
:* Transformed DB3.1 cells using 5 μl ligation reaction | :* Transformed DB3.1 cells using 5 μl ligation reaction | ||
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= Discussion = | = Discussion = | ||
Revision as of 23:13, 27 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of p+r
- Grace
- Ran RE digested products on gel
- Extracted nir, C0012 vector, BB-pRL1383a from gel
- 3A assembly (ligation) of C0012 vector (pSB1A2) with:
- nir and rbs (B0034)
- plac and rbs(B0034)
- pSB1A2 (negative control)
- Transformed DB3.1 cells using 5 μl ligation reaction
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
