Edinburgh/26 June 2008

From 2008.igem.org

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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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var tabdropdown={
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:::: '''[[Edinburgh/25_June_2008|< Previous Entry]]'''
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</script>
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<style>
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.ddcolortabs{
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width: 100%;
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background: transparent;
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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text-decoration:none;
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.ddcolortabs a span{
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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.ddcolortabs a span{
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.ddcolortabs a:hover{
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.ddcolortabs a:hover span{
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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.ddcolortabsline{
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padding: 0;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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background-color: white;
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.dropmenudiv_a a{
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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</style>
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</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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<table>
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<html>
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<style type="text/css">
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table.calendar          { margin: 0; padding: 2px; }
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table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
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table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
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table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
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#bodyContent table.month a { background:none; padding:0 }
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.day-empty  { color:#000000 }
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</style>
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</html>
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
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</table>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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== Week 2 ==
== Week 2 ==
=== Thursday 26 June 08 ===
=== Thursday 26 June 08 ===
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* Purified DNA from PCR reactions. Used 20 microlitres of glass beads and eluted to 40 microlitres of TE.
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* Purified DNA from PCR reactions. Used 20μl of glass beads and eluted to 40μl of TE.
** Sample P1: ''dxs'' PCR product
** Sample P1: ''dxs'' PCR product
** Sample P2: ''appY'' PCR product
** Sample P2: ''appY'' PCR product
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** Ligation '''L3''': ''glgC'' (5μl) + linear Babel1 (16-2-8, 2μl) with PNK
** Ligation '''L3''': ''glgC'' (5μl) + linear Babel1 (16-2-8, 2μl) with PNK
** Ligation '''L4''': ''glgC'' (5μl) + linear Babel2 (18-2-8, 2μl) with PNK<br />
** Ligation '''L4''': ''glgC'' (5μl) + linear Babel2 (18-2-8, 2μl) with PNK<br />
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ligations incubated at 16°C overnight.
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ligations incubated at 16°C overnight.<br />
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<br />
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 +
:::: '''[[Edinburgh/27_June_2008|Next Entry >]]'''

Latest revision as of 14:03, 28 August 2008

< Previous Entry

Week 2

Thursday 26 June 08

  • Purified DNA from PCR reactions. Used 20μl of glass beads and eluted to 40μl of TE.
    • Sample P1: dxs PCR product
    • Sample P2: appY PCR product
    • Sample P3: glgC PCR product
  • Set up digests to clone appY and dxs into Edinbrick1. Digests with 32μl water, 5μl buffer E, 4μl Edinbrick1 DNA, 4μl purified PCR product, 2.5μl SpeI, 2.5μl EcoRI. Incubated at 37°C. Purified. Set up ligations:
    • Ligation L1: dsx + Edinbrick1, EcoRI/SpeI
    • Ligation L2: appY + Edinbrick1, EcoRI/SpeI
    • Ligation L3: glgC (5μl) + linear Babel1 (16-2-8, 2μl) with PNK
    • Ligation L4: glgC (5μl) + linear Babel2 (18-2-8, 2μl) with PNK

ligations incubated at 16°C overnight.

Next Entry >
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