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Team:Chiba/jk/β
From 2008.igem.org
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*20 August, 2008 | *20 August, 2008 | ||
:[[Team:Chiba/protocol/DNA Purification/sigma|Sigma prep]] | :[[Team:Chiba/protocol/DNA Purification/sigma|Sigma prep]] | ||
| - | ::*BBa_C0070(2007) | + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) |
| - | ::*BBa_C0070(2006) | + | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) |
| - | ::*BBa_C0076( | + | ::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) |
| - | ::*BBa_C0078(2007) | + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) |
| - | ::*BBa_C0078(2006) | + | ::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) |
:[[Team:Chiba/protocol/digestion|Digestion]] | :[[Team:Chiba/protocol/digestion|Digestion]] | ||
| - | ::*BBa_C0170(2007) | + | ::*[http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007) |
| - | ::*BBa_C0170(2006) | + | ::*[http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006) |
| - | ::*BBa_C0178(2007) | + | ::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007) |
| - | ::*BBa_C0178(2006) | + | ::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006) |
:[[Team:Chiba/protocol/gelcheck|Gel Check]] | :[[Team:Chiba/protocol/gelcheck|Gel Check]] | ||
Revision as of 13:09, 30 August 2008
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- Transforation
- [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates
- 18 August, 2008
- Transformation
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- 19 August, 2008
- PCR
- ①[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- ②[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- ③[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- ④[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- ⑤[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- ⑥[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- ⑦[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
- Gel Check
- ①～②
- 20 August, 2008
- Sigma prep
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- Digestion
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- 21 August, 2008
- PCR
- BBa_C0070(2007)
- BBa_C0070(2006)
- BBa_C0076(2006)
- BBa_C0078(2007)
- BBa_C0078(2006)
Week 2
- 27 August, 2008
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).
Week 3
reference
- L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
- クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
- M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
- Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
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