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Team:Chiba/Calendar-Home/31 August 2008
From 2008.igem.org
(Difference between revisions)
(New page: '''OUTPUT TEAM''' *Digestion&Ligation <br>R0010(plac)+rluciferase <br>digestion <br>DNA 10μl <br>Buffer2(x10) 5μl <br>speⅠ 2μl <br>PstⅠ 2μl <br...) |
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| Line 1: | Line 1: | ||
'''OUTPUT TEAM''' | '''OUTPUT TEAM''' | ||
| - | + | <br>Digestion&Ligation | |
<br>R0010(plac)+rluciferase | <br>R0010(plac)+rluciferase | ||
<br>digestion | <br>digestion | ||
| - | <br>DNA | + | <br>DNA 10μl |
<br>Buffer2(x10) 5μl | <br>Buffer2(x10) 5μl | ||
| - | <br> | + | <br>speⅠ2μl |
| - | <br> | + | <br>PstⅠ2μl |
| - | <br>BSA | + | <br>BSA 1μl |
| - | <br>dH2O | + | <br>dH2O 30μl |
<br>--------------------- | <br>--------------------- | ||
<br> 50μl | <br> 50μl | ||
<br>->37℃ 3hours | <br>->37℃ 3hours | ||
<br>->SAP 1μl | <br>->SAP 1μl | ||
Revision as of 01:43, 1 September 2008
OUTPUT TEAM
Digestion&Ligation
R0010(plac)+rluciferase
digestion
DNA 10μl
Buffer2(x10) 5μl
speⅠ2μl
PstⅠ2μl
BSA 1μl
dH2O 30μl
---------------------
50μl
->37℃ 3hours
->SAP 1μl
