Team:Hawaii/Notebook/2008-09- 3

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(Difference between revisions)
(→Re-replacement of BB-pRL1383a MCS)
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::* BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
::* BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
::* Cut RE digested J33207 band from gel.
::* Cut RE digested J33207 band from gel.
 +
:* Overnight RE digest of BB-pRL1383a (my prep from 8/29) with HindIII and BamHI
= Discussion =
= Discussion =

Revision as of 01:09, 4 September 2008

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Things we did today

Wetlab work

Re-replacement of BB-pRL1383a MCS

Grace
EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran last night's RE digest on a gel
  • BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
  • Cut RE digested J33207 band from gel.
  • Overnight RE digest of BB-pRL1383a (my prep from 8/29) with HindIII and BamHI

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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