Latest revision as of 02:16, 6 September 2008

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Things we did today

Grace

EtBr stained 0.8% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digest reaction were loaded into each well.

BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/μl. Added 22μl for rxn (7000+ ng DNA). DNA conc. really wrong.
Cut RE digested J33207 band from gel.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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@@ Line 5: / Line 5: @@
 ===Re-replacement of BB-pRL1383a MCS===
 :<strong> Grace</strong>
-[[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.]]
+[[Image:090308REdigest.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 2 hours. Thirty microliters of RE digest reaction were loaded into each well.]]
 :* Ran last night's RE digest on a gel
-:* Extracted bands from gel
+::* BB-pRL1383a band did not show up. Nanodrop said DNA conc. ~329 ng/&mu;l. Added 22&mu;l for rxn (7000+ ng DNA). DNA conc. really wrong.
-:* Treated BB-pRL1383a w/ SAP
+::* Cut RE digested J33207 band from gel.
-:* Ligated --&mu;l J33207 w/ --&mu;l BB-pRL1383a
+:* Overnight RE digest of BB-pRL1383a (my prep from 8/29) with HindIII and BamHI
-:* Transformed DH5&alpha; cells using 5 &mu;l ligation reaction
 = Discussion =