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User:University of Washington/10 September 2008
From 2008.igem.org
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*5ul miniprep of LuxR colony2.7 (contains matching P1010) | *5ul miniprep of LuxR colony2.7 (contains matching P1010) | ||
*control: no plasmid, grew on Tsy plate | *control: no plasmid, grew on Tsy plate | ||
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| + | -found out that boiling to lyse cells yesterday was ran only for 16 s instead of 16 mins. Chances were cells weren't lysed still. Ran gel anyway on 7 of them. 1,2,3,26,28 contained nothing. 4,27 had bands around 1kb(P1010). | ||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 22:27, 10 September 2008
Glycerol Stocks of TrbA, KorA, TrbC
LuxR from AraC and TetR(Faifan)
-made 4 electro-competent MG1655Z1 from overnight x4
-re-ligated pSB3K3 + promo + LuxR
- mix 8.58ul LuxR + 2ul T4Buffer + 5ul pSB3K3 + 3.42ul promo + 1ul T4 Ligase
-transformed + inoculated onto Kan plates
- 5ul old LuxR construction ligation
- 5ul new LuxR construction ligation
- 5ul miniprep of LuxR colony2.7 (contains matching P1010)
- control: no plasmid, grew on Tsy plate
-found out that boiling to lyse cells yesterday was ran only for 16 s instead of 16 mins. Chances were cells weren't lysed still. Ran gel anyway on 7 of them. 1,2,3,26,28 contained nothing. 4,27 had bands around 1kb(P1010).
Back to Team:University_of_Washington/Notebook#Notebook
