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Team:Hawaii/Notebook/2008-09-20
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of secretion device (cont.)=== :<strong> Grace</strong> [[Image:092008REdigests.png|right|thumb|300px|EtBr...) |
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:* Ran RE digests on 4% agarose gel | :* Ran RE digests on 4% agarose gel | ||
:* Extracted bands from gel | :* Extracted bands from gel | ||
| + | :* Ligated overnight: | ||
| + | :* nir+rbs & pilA & pSB1A3 | ||
| + | :* nir+rbs & slr1+GFPf & pSB1A3 | ||
| + | :* slr1+GFPf & tt & pSB1A3 | ||
| + | :* rbs & slr1+GFPf & pSB1A3 | ||
| + | :* plac & rbs+GFP & pSB1A3 | ||
| + | :* rbs+GFP & tt & pSB1A3 | ||
| + | :* pSB1A3 to self (control) | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 02:53, 21 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on 4% agarose gel
- Extracted bands from gel
- Ligated overnight:
- nir+rbs & pilA & pSB1A3
- nir+rbs & slr1+GFPf & pSB1A3
- slr1+GFPf & tt & pSB1A3
- rbs & slr1+GFPf & pSB1A3
- plac & rbs+GFP & pSB1A3
- rbs+GFP & tt & pSB1A3
- pSB1A3 to self (control)
Drylab Work
Sequencing
- Grace & Krystle
- Figured out Krystle's sequencing results
- GFPf+tt 17F+8R = ccdB base vector
- GFPf+tt 8F+21R = rbs (B0030+GFPf)
- GFPF+tt 21F+17R = GFPf+B1006
- Need to resequence #21, 17 because no way to tell which is correct (F/R)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
