Team:BCCS-Bristol/Calendar-Notebook/21 July 2008

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== '''OD measurement''' ==
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== '''Growth curve measurement''' ==
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The OD of both strains was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.
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The OD<sub>600</sub> of ''E. coli'' MC1000 and MG1655 was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.
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A, B and C show 100, 500 and 200µl innoculations respectively.
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A, B and C show 100, 500 and 200 µl innoculations of overnight culture in 50 ml LB broth, respectively.
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| align="center" |'''Time (minutes)'''
| align="center" |'''Time (minutes)'''
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[[Image:BCCS_Bristol-wetlab-OD_measurments.JPG | 700px]]
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==Swimming agar assay==
==Swimming agar assay==
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Chemotaxis experiment for MG1655 showed no real difference btween the control (water) and the aspartate. may have been due to higher agar concentration caused by an unusually large water loss in autoclaving, causing high concentrations of agar.
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Chemotaxis experiment for MG1655 and MC1000 showed no real difference between the control (water) and the aspartate. This may have been due to higher agar concentration caused by an unusually large water loss in autoclaving, causing high concentrations of agar.
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The experiment repetition was started today.
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==BioBrick Transformation==
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Started on the transformation of bacteria with biobrick from paper, punched out hole and put in TE buffer (below) following protocol on igem sheet.  
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The transformation of bacteria with BioBrick from paper was started. Therefore, a hole was punched out of the DNA spot of a [http://partsregistry.org/Part:BBa_E0240 GFP generator] and put in TE buffer (below) following protocol on iGEM sheet.
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Chemotaxis experiment with 20% aspartate repeated.
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==Miscellaneous==
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DMSO stocks of master cultures made as back up in the -80 <sup>o</sup> C freezer.
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DMSO stocks of ''E. coli'' MC1000 and MG1655 were made as back up in the -80<sup>o</sup>C freezer.

Latest revision as of 14:01, 23 September 2008

Home The Team The Project Submitted Parts Modelling Wet Lab Calendar Miscellaneous



Growth curve measurement

The OD600 of E. coli MC1000 and MG1655 was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.

A, B and C show 100, 500 and 200 µl innoculations of overnight culture in 50 ml LB broth, respectively.


Time (minutes) OD600 (1:5 diluted)


MG1655MC1000
ABCABC
00.00160.00420.0170.00160.00440.0174
600.0080.0130.0730.0060.0170.068
800.0110.0520.1090.0090.0460.123
1000.0190.0590.1420.0170.0670.167
1200.0290.0760.1690.0240.0830.183
1400.0390.0950.2130.0390.1440.226
1600.0630.1360.260.0530.1470.275
1800.0740.1660.2760.0690.1860.298
2000.1050.2070.3010.0890.220.316
2200.1340.250.3550.1160.2680.331
2400.1780.2760.3860.1510.2930.341
2600.2330.3290.4190.1980.3250.372
2800.2390.3340.4350.2030.3260.375
3000.2630.3630.4730.2380.340.388
3200.2960.3910.4910.270.3640.412
3400.3190.4250.5110.290.3780.429
3600.320.4350.5120.2940.3820.432
3800.3920.5420.5140.3110.4340.438
4000.4350.5490.5880.3480.4570.497
4200.470.520.4870.3820.4620.504
4400.4830.5860.6330.4770.4370.516
4600.4980.4820.6290.4110.4840.517
4800.5740.6430.7210.4510.5160.524
5000.6070.660.730.4550.5140.515
5200.650.6730.7230.4530.5220.523
BCCS-080721-growth curve E. coli MC1000 and MG1655.PNG

Swimming agar assay

Chemotaxis experiment for MG1655 and MC1000 showed no real difference between the control (water) and the aspartate. This may have been due to higher agar concentration caused by an unusually large water loss in autoclaving, causing high concentrations of agar. The experiment repetition was started today.

BioBrick Transformation

The transformation of bacteria with BioBrick from paper was started. Therefore, a hole was punched out of the DNA spot of a [http://partsregistry.org/Part:BBa_E0240 GFP generator] and put in TE buffer (below) following protocol on iGEM sheet.

Miscellaneous

DMSO stocks of E. coli MC1000 and MG1655 were made as back up in the -80oC freezer.

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