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Team:Hawaii/Notebook/2008-09-24
From 2008.igem.org
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:* nrg #4, nrg #7, nrsg #8, rgt #1, rgt #2 | :* nrg #4, nrg #7, nrsg #8, rgt #1, rgt #2 | ||
:* plac+rbs | :* plac+rbs | ||
| + | ::* PCR machine froze while ExoSAPing. Unable to retrieve samples from machine. Jamie says she will figure it out. | ||
= Discussion = | = Discussion = | ||
Revision as of 00:25, 25 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verified plasmid preps
- Grace
- PCR of plasmid preps from yesterday to verify inserts
- Ran PCR products on gel
- nrg #1 and nrsg #2 returned blanks. Bad plasmid prep?
- BB-pRL1383a and colony PCR of BB-pRL1383a E. coli both blank. Yikes!
- Quantified BB-pRL1383a and pSB1A3 plasmid preps
- Blanks. Really low conc.? Will nanodrop to check.
Construction of secretion device (cont.)
- Grace
- Overnight RE digest:
- nir, nrsg, and nrg PCR products with EcoRI and SpeI
- rgt PCR products with XbaI and PstI
- J33207 PCR product and pRL1383a plasmid with HindIII and BamHI
Prep for sequencing
- Grace
- nrg #4, nrg #7, nrsg #8, rgt #1, rgt #2
- plac+rbs
- PCR machine froze while ExoSAPing. Unable to retrieve samples from machine. Jamie says she will figure it out.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
