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Purdue/25 September 2008
From 2008.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
**5uL LacZ DNA | **5uL LacZ DNA | ||
**12uL SOS DNA | **12uL SOS DNA | ||
| - | *Let sit for | + | *Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up) |
| - | *Heat inactivated enzyme for | + | *Heat inactivated enzyme for 20 min at 55C |
'''Edited by Janie Stine''' | '''Edited by Janie Stine''' | ||
Revision as of 17:25, 25 September 2008
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Performed ligation reaction with LacZ and SOS:
- Mixture:
- 1uL T4 ligase
- 2uL T4 buffer
- 5uL LacZ DNA
- 12uL SOS DNA
- Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up)
- Heat inactivated enzyme for 20 min at 55C
Edited by Janie Stine
