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| - | >[[Team:Chiba/jk/β|送受信班]]
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| | | | |
| - | ==Week 1==
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| - |
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| - | ===17 August, 2008===
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| - | :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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| - | :competent cells : XL10G
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| - | ::*BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
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| - | ::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
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| - | ::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
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| - | ::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
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| - | ::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
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| - | ::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates
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| - |
| |
| - | ===18 August, 2008===
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| - | :'''[[Team:Chiba/protocol/PCR|PCR]]'''
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| - | ::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070]
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| - | ::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076]
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| - | ::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077]
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| - | ::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078]
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| - | ::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079]
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| - | ::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
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| - |
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| - |
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| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">DNA Template</td>
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| - | <td>1</td>
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| - | </tr>
| |
| - | <tr>
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| - | <td>dNTP mix</td>
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| - | <td>10</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Foward Primer</td>
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| - | <td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Reverse Primer</td>
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| - | <td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>DNA polymerase</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Thermopol Buffer</td>
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| - | <td>10</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>68</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>100μL</td>
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| - | </tr>
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| - | </table>
| |
| - |
| |
| - |
| |
| - | :95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
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| - |
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| - |
| |
| - |
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| - | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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| - |
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| - |
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| - | {|align="justify"
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| - | |[[Image:Chiba-0818.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>3</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>7</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>12μL</td>
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| - | </tr>
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| - | </table>
| |
| - |
| |
| - | :From left;
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| - | ::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] ->None
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| - | ::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] ->None
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| - | ::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] ->None
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| - | ::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] ->None
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| - | ::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] ->None
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| - | ::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK
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| - | |}
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| - |
| |
| - |
| |
| - |
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| - |
| |
| - | :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
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| - | competent cells : XL10G
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| - |
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| - | ::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
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| - | ::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
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| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
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| - | ::*BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
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| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
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| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
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| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
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| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
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| - |
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| - |
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| - | :--->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
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| - |
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| - | ===19 August, 2008===
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| - | :'''[[Team:Chiba/protocol/PCR|PCR]]'''
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| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
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| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
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| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
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| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
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| - | ::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
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| - | ::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
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| - | ::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
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| - |
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| - |
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| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">DNA Template</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dNTP mix</td>
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| - | <td>10</td>
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| - | </tr>
| |
| - | <tr>
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| - | <td>Foward Primer</td>
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| - | <td>5</td>
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| - | </tr>
| |
| - | <tr>
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| - | <td>Reverse Primer</td>
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| - | <td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>DNA polymerase VENT</td>
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| - | <td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Thermopol Buffer</td>
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| - | <td>5</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>28</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>50μL</td>
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| - | </tr>
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| - | </table>
| |
| - |
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| - |
| |
| - | :95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
| |
| - |
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| - |
| |
| - |
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| - | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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| - | {|align="justify"
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| - | |[[Image:Chiba-0819.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>3</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>7</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>12μL</td>
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| - | </tr>
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| - | </table>
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| - |
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| - | :From left;
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| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
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| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
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| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
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| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
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| - | ::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
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| - | ::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
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| - | ::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK
| |
| - | |}
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| - |
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| - |
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| - |
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| - | ===20 August, 2008===
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| - | :(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
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| - | ::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
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| - | ::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
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| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
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| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
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| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
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| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
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| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
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| - |
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| - |
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| - | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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| - | {|align="justify"
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| - | |[[Image:Chiba-0820-3.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>3</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>7</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>12μL</td>
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| - | </tr>
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| - | </table>
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| - |
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| - | :From left;
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| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
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| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
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| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
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| - | |}
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| - |
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| - |
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| - |
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| - | {|align="justify"
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| - | |[[Image:Chiba-0820-4.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>10</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>4</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>10</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>24μL</td>
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| - | </tr>
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| - | </table>
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| - |
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| - | :From left;
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| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
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| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
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| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
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| - | ::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
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| - | ::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
| |
| - | |}
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| - |
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| - |
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| - |
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| - |
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| - | {|align="justify"
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| - | |[[Image:Chiba-0820.JPG]]
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| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>3</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Loading Dye</td>
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| - | <td>2</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>7</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>12μL</td>
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| - | </tr>
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| - | </table>
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| - |
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| - | :From left;<br>
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| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
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| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
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| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
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| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
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| - | |}
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| - |
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| - |
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| - | --->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
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| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
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| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
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| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
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| - |
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| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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| - | <tr>
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| - | <td width="257">Sample No</td>
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| - | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]</td>
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| - | <td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]</td>
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| - | <td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]</td>
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| - | <td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]</td>
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| - | <td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
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| - | </tr>
| |
| - | <tr>
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| - | <td>EcoRⅠ</td>
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| - | <td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>PstⅠ</td>
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| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>Buffer EcoRⅠ</td>
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| - | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>BSA</td>
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| - | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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| - | </tr>
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| - | <tr>
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| - | <td>dH2O</td>
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| - | <td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
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| - | </tr>
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| - | <tr>
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| - | <td>TOTAL</td>
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| - | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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| - | </tr>
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| - | </table>
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| - |
| |
| - |
| |
| - |
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| - | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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| - | {|align="justify"
| |
| - | |[[Image:Chiba-0820-2.JPG]]
| |
| - | |
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| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
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| - | <td width="257">Sample DNA</td>
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| - | <td>3</td>
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| - | </tr>
| |
| - | <tr>
| |
| - | <td>Loading Dye</td>
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| - | <td>2</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>7</td>
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| - | </tr>
| |
| - | <tr>
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| - | <td>TOTAL</td>
| |
| - | <td>12μL</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | :From left;
| |
| - | :(Single Digestion 1~4)
| |
| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
| |
| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
| |
| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
| |
| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
| |
| - | :(Double Digestion 1~4)
| |
| - | ::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
| |
| - | ::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
| |
| - | ::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
| |
| - | ::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
| |
| - | |}
| |
| - |
| |
| - | ===21 August, 2008===
| |
| - | :(20/8)--->'''[[Team:Chiba/protocol/PCR|PCR]]'''
| |
| - | ::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
| |
| - | ::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
| |
| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
| |
| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
| |
| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
| |
| - |
| |
| - |
| |
| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">DNA Template</td>
| |
| - | <td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dNTP mix</td>
| |
| - | <td>10</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Foward Primer</td>
| |
| - | <td>5</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Reverse Primer</td>
| |
| - | <td>5</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>DNA polymerase TAQ</td>
| |
| - | <td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Thermopol Buffer</td>
| |
| - | <td>5</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>28</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>50μL</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - | :95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
| |
| - |
| |
| - |
| |
| - |
| |
| - | :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
| |
| - | {|align="justify"
| |
| - | |[[Image:Chiba-0821.JPG]]
| |
| - | |
| |
| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">Sample DNA</td>
| |
| - | <td>3</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Loading Dye</td>
| |
| - | <td>2</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>7</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>12μL</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - | :From left;
| |
| - | ::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
| |
| - | ::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
| |
| - | ::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
| |
| - | ::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
| |
| - | ::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - | :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
| |
| - | :competent cells : XL10G
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | --->(23/8)'''[[Team:Chiba/protocol/digestion|Digestion]]'''
| |
| - |
| |
| - |
| |
| - |
| |
| - | ===22 August, 2008===
| |
| - | :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
| |
| - | :competent cells : XL10G
| |
| - | ::*BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
| |
| - | ::*BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
| |
| - | ::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
| |
| - | ::*BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
| |
| - | :(Locate & 2 Punch ~iyama ver.~)
| |
| - | ::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
| |
| - | ::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
| |
| - | ::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
| |
| - | ::*BBa_J04500(2008) [http://partsregistry.org/Part:BBa_J04500] -> No colonies on plates
| |
| - |
| |
| - |
| |
| - |
| |
| - | --->(24/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
| |
| - |
| |
| - | ===23 August, 2008===
| |
| - | :(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
| |
| - |
| |
| - |
| |
| - | :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
| |
| - |
| |
| - |
| |
| - | :<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="270">Sample No</td>
| |
| - | <td> 1</td><td> 2</td><td> 3</td><td> 4</td><td> 5</td><td> 6</td><td> 7</td><td> 8</td><td> 9</td><td>10</td><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td><td>21</td>
| |
| - | </tr><tr>
| |
| - | <td>BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]</td>
| |
| - | <td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]</td>
| |
| - | <td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]</td>
| |
| - | <td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <tr>
| |
| - | <td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td></tr>
| |
| - | <tr>
| |
| - | <td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td></tr>
| |
| - | <tr>
| |
| - | <td>BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td></tr>
| |
| - | <tr>
| |
| - | <td>EcoRⅠ</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>PstⅠ</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Buffer EcoRⅠ</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>BSA</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>1</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - |
| |
| - | :--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
| |
| - | {|align="justify"
| |
| - | |[[Image:Chiba-0823-1.JPG]]
| |
| - | |
| |
| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">Sample No</td>
| |
| - | <td>1~6</td><td>7</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Sample DNA</td>
| |
| - | <td>3</td><td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Loading Dye</td>
| |
| - | <td>1</td><td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>2</td><td>4</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>6</td><td>6</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | :From left; Sanple No. 1~7
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
| |
| - | ::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | {|align="justify"
| |
| - | |[[Image:Chiba-0823-2.JPG]]
| |
| - | |
| |
| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">Sample No</td>
| |
| - | <td>8~21</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Sample DNA</td>
| |
| - | <td>10</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Loading Dye</td>
| |
| - | <td>2</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>12</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - | :From left;
| |
| - | :(Single Digestion) Sanple No. 8~14
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
| |
| - | ::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
| |
| - |
| |
| - | :(Double Digestion) Sample No. 15~21
| |
| - | ::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
| |
| - | ::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
| |
| - | ::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
| |
| - | ::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
| |
| - | ::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
| |
| - | ::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
| |
| - | ::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | :'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
| |
| - | ::BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
| |
| - |
| |
| - | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">Sample No</td>
| |
| - | <td>1</td><td>2</td><td>3</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Sample DNA</td>
| |
| - | <td>3</td><td>3</td><td>3</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>EcoRⅠ</td>
| |
| - | <td>-</td><td>0.1</td><td>0.1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>SpeⅠ</td>
| |
| - | <td>-</td><td>-</td><td>0.1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>EcoRⅠ Buffer</td>
| |
| - | <td>-</td><td>0.9</td><td>0.8</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>BSA</td>
| |
| - | <td>-</td><td>1</td><td>1</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>-</td><td>5</td><td>5</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>3</td><td>10</td><td>10</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - |
| |
| - |
| |
| - | --->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
| |
| - | {|align="justify"
| |
| - | |[[Image:Chiba-0823-3.JPG]]
| |
| - | |
| |
| - | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
| |
| - | <tr>
| |
| - | <td width="257">Sample No</td>
| |
| - | <td>1</td><td>2</td><td>3</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Sample DNA</td>
| |
| - | <td>3</td><td>10</td><td>10</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>Loading Dye</td>
| |
| - | <td>1</td><td>2</td><td>2</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>dH2O</td>
| |
| - | <td>2</td><td>-</td><td>-</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td>TOTAL</td>
| |
| - | <td>6</td><td>12</td><td>12</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | :From left;
| |
| - | ::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
| |
| - | ::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
| |
| - | ::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
| |
| - | |}
| |
"
