Judging/Variance/LCG-UNAM Mexico

From 2008.igem.org

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    Dear iGEM judges,
 +
    The team LCG-UNAM-MEXICO is participating this year for the first
 +
    time, and I'm writing on behalf of the team to request permission
 +
    for submitting our Biobricks in non-standard vectors.
 +
 +
 +
    Our project requires very tight regulation of E. coli nickel
 +
    extrusion pump (RcnA) through several regulatory upstream proteins.
 +
    Our Analysis suggest that we need to control very accurately the
 +
    production of several molecules and a crucial part of this requires
 +
    stable low copy-number vectors. For this reason we would like to use
 +
    pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will
 +
    be adding Biobrick prefixx and suffix to both of them. We will also
 +
    make sure that our parts are easily extracted from the plasmid
 +
    either through digestion with commercially available enzymes or
 +
    through PCR.
 +
 +
 +
 +
 +
    All this information will be shared to ensure that our Biobricks are
 +
    usefull for the community.
 +
 +
 +
 +
 +
    Thanks for your time and consideration
 +
 +
 +
 +
 +
    Sur Herrera Paredes
 +
    LCG-UNAM-MEXICO
 +
    Licenciatura en Ciencias Genómicas
 +
    Universidad Nacional Autónoma de México

Revision as of 15:39, 30 September 2008

    Dear iGEM judges,

    The team LCG-UNAM-MEXICO is participating this year for the first
    time, and I'm writing on behalf of the team to request permission
    for submitting our Biobricks in non-standard vectors. 



    Our project requires very tight regulation of E. coli nickel
    extrusion pump (RcnA) through several regulatory upstream proteins.
    Our Analysis suggest that we need to control very accurately the
    production of several molecules and a crucial part of this requires
    stable low copy-number vectors. For this reason we would like to use
    pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will
    be adding Biobrick prefixx and suffix to both of them. We will also
    make sure that our parts are easily extracted from the plasmid
    either through digestion with commercially available enzymes or
    through PCR.




    All this information will be shared to ensure that our Biobricks are
    usefull for the community.




    Thanks for your time and consideration




    Sur Herrera Paredes
    LCG-UNAM-MEXICO
    Licenciatura en Ciencias Genómicas
   Universidad Nacional Autónoma de México
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