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Judging/Variance/LCG-UNAM Mexico
From 2008.igem.org
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Dear iGEM judges, | Dear iGEM judges, | ||
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The team LCG-UNAM-MEXICO is participating this year for the first | The team LCG-UNAM-MEXICO is participating this year for the first | ||
time, and I'm writing on behalf of the team to request permission | time, and I'm writing on behalf of the team to request permission | ||
for submitting our Biobricks in non-standard vectors. | for submitting our Biobricks in non-standard vectors. | ||
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Our project requires very tight regulation of E. coli nickel | Our project requires very tight regulation of E. coli nickel | ||
extrusion pump (RcnA) through several regulatory upstream proteins. | extrusion pump (RcnA) through several regulatory upstream proteins. | ||
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either through digestion with commercially available enzymes or | either through digestion with commercially available enzymes or | ||
through PCR. | through PCR. | ||
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All this information will be shared to ensure that our Biobricks are | All this information will be shared to ensure that our Biobricks are | ||
usefull for the community. | usefull for the community. | ||
Revision as of 15:41, 30 September 2008
Dear iGEM judges,
The team LCG-UNAM-MEXICO is participating this year for the first
time, and I'm writing on behalf of the team to request permission
for submitting our Biobricks in non-standard vectors.
Our project requires very tight regulation of E. coli nickel
extrusion pump (RcnA) through several regulatory upstream proteins.
Our Analysis suggest that we need to control very accurately the
production of several molecules and a crucial part of this requires
stable low copy-number vectors. For this reason we would like to use
pRK415 and pBBIMCS-5 derivatives as our plasmid backbones. We will
be adding Biobrick prefixx and suffix to both of them. We will also
make sure that our parts are easily extracted from the plasmid
either through digestion with commercially available enzymes or
through PCR.
All this information will be shared to ensure that our Biobricks are
usefull for the community.
Thanks for your time and consideration
Sur Herrera Paredes
LCG-UNAM-MEXICO
Licenciatura en Ciencias Genómicas
Universidad Nacional Autónoma de México
