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Team:Warsaw/Calendar-Main/11 June 2008
From 2008.igem.org
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<p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7. | <p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7. | ||
Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p> | Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p> | ||
| - | + | Rifampicin test of transformants | |
Revision as of 17:31, 1 October 2008
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Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal). Rifampicin test of transformants
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