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Team:Warsaw/Calendar-Main/24 July 2008
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<p>Paweł: <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Plasmid DNA isolation</a> from overnight grown cultures. Restriction analysis (BamHI+NdeI) of 5 of them</p> | <p>Paweł: <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Plasmid DNA isolation</a> from overnight grown cultures. Restriction analysis (BamHI+NdeI) of 5 of them</p> | ||
<p>Results: 2 of analysed plasmids contain OmpA+omega (~1350 bp band)</p> | <p>Results: 2 of analysed plasmids contain OmpA+omega (~1350 bp band)</p> | ||
| - | <p>First of positive plasmids and Z (in GeneArt vector) digest with NdeI and NotI (overnight)< | + | <p>First of positive plasmids (now called pET15b+OmpA+omega) and Z (in GeneArt vector) digest with NdeI and NotI (overnight)</p> |
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1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin. Paweł: Plasmid DNA isolation from overnight grown cultures. Restriction analysis (BamHI+NdeI) of 5 of them Results: 2 of analysed plasmids contain OmpA+omega (~1350 bp band) First of positive plasmids (now called pET15b+OmpA+omega) and Z (in GeneArt vector) digest with NdeI and NotI (overnight)
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