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Team:Warsaw/Calendar-Main/24 July 2008
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1. Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br> | 1. Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br> | ||
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| + | <h3>Cloning of protein A DNA to pET15b+Omp-alfa plasmid<br>Antoni</h3> | ||
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| + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylation with CIAP</a> </li> </li> | ||
| + | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | ||
| + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+alfa and A </li> | ||
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| + | </ol></p> | ||
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Revision as of 20:20, 2 October 2008
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1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin. Antoni:
1. Preparation of chemocompetent bacteria E. coli TOP10. Cloning of protein A DNA to pET15b+Omp-alfa plasmid
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