Team:Warsaw/Calendar-Main/24 July 2008
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<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands </li> | ||
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+alfa and A </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+alfa and A </li> | ||
- | </li> | + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a>. </li> |
+ | <li> Transformants plating on LB + ampitilin.</li> | ||
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</ol></p> | </ol></p> | ||
Revision as of 20:22, 2 October 2008
1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin. Antoni:
1. Preparation of chemocompetent bacteria E. coli TOP10. Cloning of protein A DNA to pET15b+Omp-alfa plasmid
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