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Team:Hawaii/Notebook/2008-09-20
From 2008.igem.org
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===Construction of secretion device (cont.)=== | ===Construction of secretion device (cont.)=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | [[Image:092008REdigests.png|right|thumb| | + | [[Image:092008REdigests.png|right|thumb|600px|EtBr stained 4% agarose gels ran at 60V for 2.5 hours. Twenty five microliters of RE digest were loaded into each well.]] |
:* Ran RE digests on 4% agarose gel | :* Ran RE digests on 4% agarose gel | ||
:* Extracted bands from gel | :* Extracted bands from gel | ||
Latest revision as of 20:58, 3 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Ran RE digests on 4% agarose gel
- Extracted bands from gel
- Ligated overnight:
- nir+rbs & pilA & pSB1A3
- nir+rbs & slr1+GFPf & pSB1A3
- slr1+GFPf & tt & pSB1A3
- rbs & slr1+GFPf & pSB1A3
- plac & rbs+GFP & pSB1A3
- rbs+GFP & tt & pSB1A3
- pSB1A3 to self (control)
Drylab Work
Sequencing
- Grace & Krystle
- Figured out Krystle's sequencing results
- GFPf+tt 17F+8R = ccdB base vector
- GFPf+tt 8F+21R = rbs (B0030+GFPf)
- GFPF+tt 21F+17R = GFPf+B1006
- Need to resequence #21, 17 because no way to tell which is correct (F/R)
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
