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Edinburgh/18 August 2008
From 2008.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
* Minipreps: | * Minipreps: | ||
| - | '''M144-M147:''' Minipreps of cenA(1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.<br> | + | **'''M144-M147:''' Minipreps of cenA(1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.<br> |
| - | '''M148-M151:''' Minipreps of cex(1,2,3,4).<br> | + | **'''M148-M151:''' Minipreps of cex(1,2,3,4).<br> |
| - | '''M152-M155:''' Minipreps of dxs-crtE (1,2,3,4).(OG) | + | **'''M152-M155:''' Minipreps of dxs-crtE (1,2,3,4).(OG) |
* Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH) | * Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH) | ||
* Maxipreps: | * Maxipreps: | ||
| - | '''X11:''' Zea mays isoamylase 1 (isa1+pZntr22).<br> | + | **'''X11:''' Zea mays isoamylase 1 (isa1+pZntr22).<br> |
| - | '''X12:''' Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX) | + | **'''X12:''' Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX) |
* Combined biobricks: | * Combined biobricks: | ||
| - | '''L42:''' dxs+lims (M137) to appY (M82).<br> | + | **'''L42:''' dxs+lims (M137) to appY (M82).<br> |
| - | '''L43:''' crtB+crtI (M109) to appY (M82).<br> | + | **'''L43:''' crtB+crtI (M109) to appY (M82).<br> |
| - | '''L44:''' pstA (M130) to lacB. (YAN) | + | **'''L44:''' pstA (M130) to lacB. (YAN) |
* Blunt-ended ligations '''L45~L47''' of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM) | * Blunt-ended ligations '''L45~L47''' of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM) | ||
* Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM) | * Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM) | ||
'''[[Edinburgh/19_August_2008|Next Entry >]]''' | '''[[Edinburgh/19_August_2008|Next Entry >]]''' | ||
Revision as of 16:38, 6 October 2008
Week 10
Monday 18 August 08
- Minipreps:
- M144-M147: Minipreps of cenA(1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.
- M148-M151: Minipreps of cex(1,2,3,4).
- M152-M155: Minipreps of dxs-crtE (1,2,3,4).(OG)
- M144-M147: Minipreps of cenA(1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.
- Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)
- Maxipreps:
- X11: Zea mays isoamylase 1 (isa1+pZntr22).
- X12: Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)
- X11: Zea mays isoamylase 1 (isa1+pZntr22).
- Combined biobricks:
- L42: dxs+lims (M137) to appY (M82).
- L43: crtB+crtI (M109) to appY (M82).
- L44: pstA (M130) to lacB. (YAN)
- L42: dxs+lims (M137) to appY (M82).
- Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
- Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
