Team:Warsaw/Calendar-Main/20 June 2008

From 2008.igem.org

(Difference between revisions)
Line 24: Line 24:
</table>
</table>
-
<p>Polymerase: homemade Pfu-Turbo</p>
+
<p>Polymerase: Pfu-Turbo</p>
</html>
</html>

Revision as of 21:15, 7 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Michał L., Ewa, Marcin

PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 + AP+NotI 470 bp
alpha-linkerpUC19AlphaL+SacI + AlphaP+link10+homo2 1022 bp
omega-linkerpUC19OmegaL+SacI + OmegaP+link10+homo2 779 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: Pfu-Turbo