Team:Warsaw/Calendar-Main/20 June 2008

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<h3>Preparation of constructs with OmpA protein fusions</h3>
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<h4>Michał K.:</h4>
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Revision as of 14:44, 8 October 2008

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Michał L., Ewa, Marcin:

PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 and AP+NotI 470 bp
alpha-linkerpUC19 AlphaL+SacI and AlphaP+link10+homo2600 bp
omega-linkerpUC19OmegaL+SacI and OmegaP+link10+homo2400 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: Pfu-Turbo

Preparation of constructs with OmpA protein fusions

Michał K.: