Team:Warsaw/Calendar-Main/6 June 2008

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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li>
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<li>DNA gel electrophoresis.</li>
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<li>DNA gel electrophoresis.</li></ol>
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Revision as of 14:54, 8 October 2008

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Michał L., Ewa, Marcin:

  1. Making chemocompetent E. coli TOP10 carrying pZC320.

Preparation of pMPMT5-AID+AIDT7 construct

Michał K.:

  1. Isolation of hypothetical pMPM-T5-AID+AIDT7.
  2. Control Digest of pMPM-T5-AID+AIDT7 with BamHI.
  3. DNA gel electrophoresis.

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