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Team:ESBS-Strasbourg/8 October 2008
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== WetLab == | == WetLab == | ||
| + | |||
| + | '''Katja:''' <br\n> | ||
| + | <br\n> | ||
| + | ''results of the last two days:''<br\n> | ||
| + | We have | ||
| + | * mCherry_L_Vp16_L_lexA_L | ||
| + | * CFP_cin8_adh1term (Manuels work) | ||
| + | * Gal1_CFP_NLS_adh1term | ||
| + | * Gal1_CFP_hsl1_adhterm | ||
| + | * Gal1_mCherrry_L_lexA_L_VP16_L<br\n> | ||
| + | <br\n> | ||
| + | So further assemblies will be: | ||
| + | * Gal1 (BV - in the freezer) + mCherry_L_Vp16_L_lexA_L (BI - to be done) | ||
| + | * Gal1 (BV - in the freezer) + lexA_L_Vp16_L_mCherry_L (BI - to be done) | ||
| + | * Gal1 (BV - in the freezer) + CFP_cln2_adhterm (BI - to be remake) | ||
| + | * Gal1 (BV - in the freezer) + CFP_cin8-adhterm (BI - to be done) | ||
| + | * Gal1_mCherry_L_lexA_L_Vp16_L (FI - to be done) + NLS_adhterm (FV - to be done) | ||
| + | * Gal1_mCherry_L_lexA_L_Vp16_L (BV - to be done) + NLS_adhterm (BI - to be done) (for savety) | ||
| + | |||
| + | * Leu2 (BI) in pSB1A2 (X+P) | ||
| + | * Ura3 (FI) in pSB1A2 (E+S) | ||
| + | <br\n> | ||
== General == | == General == | ||
Latest revision as of 18:50, 8 October 2008
Contents |
DryLab
Modeling
WetLab
Katja:
results of the last two days:
We have
- mCherry_L_Vp16_L_lexA_L
- CFP_cin8_adh1term (Manuels work)
- Gal1_CFP_NLS_adh1term
- Gal1_CFP_hsl1_adhterm
- Gal1_mCherrry_L_lexA_L_VP16_L
So further assemblies will be:
- Gal1 (BV - in the freezer) + mCherry_L_Vp16_L_lexA_L (BI - to be done)
- Gal1 (BV - in the freezer) + lexA_L_Vp16_L_mCherry_L (BI - to be done)
- Gal1 (BV - in the freezer) + CFP_cln2_adhterm (BI - to be remake)
- Gal1 (BV - in the freezer) + CFP_cin8-adhterm (BI - to be done)
- Gal1_mCherry_L_lexA_L_Vp16_L (FI - to be done) + NLS_adhterm (FV - to be done)
- Gal1_mCherry_L_lexA_L_Vp16_L (BV - to be done) + NLS_adhterm (BI - to be done) (for savety)
- Leu2 (BI) in pSB1A2 (X+P)
- Ura3 (FI) in pSB1A2 (E+S)
General
Katja:
I ordered primers for the Mutagenesis of Leu2 (silent mutation) and Ura3 (in non coding region) so that we can deleted the EcoRI and Pst1 site respectively.
According to Mr. Chatton there shouldn't be a problem with transcriptional/translational regulation at this point.
