Team:Warsaw/Calendar-Main/24 May 2008

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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7<br>
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<h4>Michał K.:</h4>
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Michał K.</h3>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li>
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</li>
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<li> Gel electrophoresis (still no proper colonies found).</li></html>
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<li> Gel electrophoresis (still no proper colonies found).</li>
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Revision as of 14:38, 9 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7
Michał K.

  1. Isolation of hypothetical pMPM-T5 with transcription fusion from other colonies.
  2. Isolation of hypothetical pMPM-T5 with translation fusion from other colonies.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI (EcoRI buffer).
  4. Control digest of pMPM-T5 with translation fusion with BamHI (BamHI buffer).
  5. Gel electrophoresis (still no proper colonies found).

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