Team:Warsaw/Calendar-Main/17 June 2008

(Difference between revisions)

Revision as of 15:10, 9 October 2008


	Attribution analyzer / Interactive list of topics

PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega.
Electrophoresis to estimate the concentration of isolated DNA.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.
Inoculation of E. coli TOP10 with pACYC177 plasmid into liquid LB + kanamycin.

Repetition of experiment from 11/06/2008.

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 <html>
-<h4>Michał L., Ewa, Marcin:</h4>
-<ol>
-<li>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></li></ol><br>
-<h3>Preparation of constructs with OmpA protein fusions</h3>
+<h3>Preparation of constructs with OmpA protein fusions<br>
-<h4>Michał K.</h4>
+Michał K.</h3>
 <p><ol>
 <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on OmpA_linker and linker_alpha  with
@@ Line 26: / Line 23: @@
 <li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI. </li>
 <li> Inoculation of  <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with pACYC177 plasmid into liquid LB + kanamycin. </li>
-</ol></html>
+</ol></p>
+<h4>Michał L., Ewa, Marcin:</h4>
+<p>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></p>
+</html>
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