Team:Warsaw/Calendar-Main/20 June 2008

(Difference between revisions)

Revision as of 15:23, 9 October 2008


	Attribution analyzer / Interactive list of topics

PCR
Product	Template	Primers	Product length
linker-A	pDRIVE-TapTag	AL+link10+homo2 and AP+NotI	470 bp
alpha-linker	pUC19	AlphaL+SacI and AlphaP+link10+homo2	600 bp
omega-linker	pUC19	OmegaL+SacI and OmegaP+link10+homo2	400 bp

Polymerase: Pfu-Turbo

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+<h3>Preparation of constructs with OmpA protein fusions<br>
+Michał K.</h3>
+<ol>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li>
+<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with PstI (Orange buffer).</li>
+<li>Gel electrophoresis (no proper colonies founded).</li></ol>
 <h4>Michał L., Ewa, Marcin:</h4>
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 </table>
-<h3>Universal PCR program for A, alpha and omega</h3>
+<h4 style="test-align: center">Universal PCR program for A, alpha and omega</h4>
 <table id="result">
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 <p>Polymerase: Pfu-Turbo</p>
-<h3>Preparation of constructs with OmpA protein fusions</h3>
-<h4>Michał K.:</h4>
-<ol>
-<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li>
-<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of isolated plasmids with PstI (Orange buffer).</li>
-<li>Gel electrophoresis (no proper colonies founded).</li></ol>
 </html>