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Purdue/25 September 2008
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| + | Performed ligation reaction with LacZ and SOS: | ||
| + | *Mixture: | ||
| + | **1uL T4 ligase | ||
| + | **2uL T4 buffer | ||
| + | **5uL LacZ DNA | ||
| + | **12uL SOS DNA | ||
| + | *Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up) | ||
| + | *Heat inactivated enzyme for 20 min at 55C | ||
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| + | *Craig, involved in learning Matlab, forgot about the enzyme. The water bath was at 58C when he took the enzyme out at 2:45PM. Heat inactivation for 1 hour, 15 minutes. | ||
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| + | '''Edited by Janie Stine''' | ||
Latest revision as of 17:51, 9 October 2008
Click Here to return to the notebook.
Performed ligation reaction with LacZ and SOS:
- Mixture:
- 1uL T4 ligase
- 2uL T4 buffer
- 5uL LacZ DNA
- 12uL SOS DNA
- Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up)
- Heat inactivated enzyme for 20 min at 55C
- Craig, involved in learning Matlab, forgot about the enzyme. The water bath was at 58C when he took the enzyme out at 2:45PM. Heat inactivation for 1 hour, 15 minutes.
Edited by Janie Stine
