Team:Warsaw/Calendar-Main/11 June 2008

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<html><h4>Michał L., Ewa, Marcin:</h4>
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<h3> Blue/white test </h3><h4>Michał L., Ewa, Marcin:</h4>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:

Revision as of 20:32, 9 October 2008

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Blue/white test

Michał L., Ewa, Marcin:

  1. Transformation of E. coli TOP10 carrying pZC320 with:
    • pMPMT5,
    • pMPMT5-AID,
    • pMPMT5-AIDT7,
    • pMPMT5-AID+T7.
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Rifampicin test of transformants.

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