Team:Freiburg/Project

From 2008.igem.org

(Difference between revisions)

Revision as of 23:11, 9 October 2008

Project Report

_projects

under construction

Modular Synthetic Receptor System

Schematical overview of the system

Abstract:

Signaling through membranes is a characteristic of life. Transmembrane proteins control proliferation, differentiation, and cellular response and are key for the formation of multicellular organisms. Controlling such proteins enables modifying cellular behavior and ultimately programming cells at will. The complex rules for transmembrane signaling often require engagement of several proteins in a fine-tuned spatial and temporal manner.

To tap possibilities of transmembrane programming, the Freiburg 2008 iGEM team provides an extensible system comprising an external framework with spatial resolution, a concept for modifying natural receptors, and a modular set of fusion-Biobricks for the construction of synthetic receptors. Spatial resolution in the nanometer scale is provided by DNA-Origami modified with distinct patterns and combinations of ligands. Receptors are decoupled from their natural ligands by fusion with artificial binding domains. The Biobrick collection contains signal sequences, binding domains, transmembrane domains, and effector domains featuring split enzymes and split fluorescent proteins for immediate readout. Project Report

@@ Line 10: / Line 10: @@
 <br>
 '''Abstract:'''<br>
-This year´s main project is the attempt to create an "artificial receptor-system", featuring extra- and intracellular modules as well as suitable transmembrane regions.
-The intracellular domaine of our receptor-device is build by halves of split reporter-proteins that can reassemble and will then produce readable output, e. g. fluorescence.
+Signaling through membranes is a characteristic of life. Transmembrane proteins control proliferation, differentiation, and cellular response and are key for the formation of multicellular organisms. Controlling such proteins enables modifying cellular behavior and ultimately programming cells at will. The complex rules for transmembrane signaling often require engagement of several proteins in a fine-tuned spatial and temporal manner.
-Each one of these protein-halves is connected to its extracellular domaine by a single-span transmembrane-helix.
-The extracellular or detecting domaine consists of a protein or peptide with the ability to bind a certain molecule.<br>
+To tap possibilities of transmembrane programming, the Freiburg 2008 iGEM team provides an extensible system comprising an external framework with spatial resolution, a concept for modifying natural receptors, and a modular set of fusion-Biobricks for the construction of synthetic receptors. Spatial resolution in the nanometer scale is provided by DNA-Origami modified with distinct patterns and combinations of ligands. Receptors are decoupled from their natural ligands by fusion with artificial binding domains. The Biobrick collection contains signal sequences, binding domains, transmembrane domains, and effector domains featuring split enzymes and split fluorescent proteins for immediate readout.
-Now, if a system with two matching receptors is presented these molecules in a strict, pairwise spatial arrangement, the receptor-devices are brought together,
-the split reporter-protein reassembles inside the cell and the output can be detected.
-We employ so-called "Origami-DNA" to create the exactly defined molecule-patterns that are needed to activate our receptors.
 [[Modular Synthetic  Receptor System|Project Report]]
 <br><br>